基于基因组改组技术选育多重抗性乙醇酵母.pdfVIP

现代食品科技 Modern Food Science and Technology 2009, Vol.25, No.10 基于基因组改组技术选育多重抗性乙醇酵母 梁磊1,2 2 2 2 2 1 ，张远平 ，黄向阳 ，徐日益 ，尚红岩 ，梁世中 （1.华南理工大学生物科学与工程学院，广州 510000）（2.广州甘蔗糖业研究所，广州510316） 摘要：本文通过基因组改组(Genome shuffling)技术将酵母菌株的多重耐受性能集中于同一菌株，从而选育出多重抗性的乙醇酵 母。以分别经过热灭活和紫外灭活的酵母 GZ-0533、GZ-241、GZ-582 为亲本菌株，经过 3 轮基因改组后，筛选得到的 28 株酵母菌。 第二次改组得到的MSR14 和第三次改组得到的 MSR23 对多重胁迫条件的耐受性提高最多，乙醇产量分别达到8.58%和 8.86%，较多 重抗性最好的出发菌 GZ-05 相对提高了 7%和 10.5%。26SrDNA 序列分析表明，MSR14 和 MSR23 的 26SrDNA 序列与酿酒酵母 （Saccharomyces cerevisiae ）的同源性为100%，均认定为酿酒酵母。 关键词：基因组改组技术；多重抗性乙醇酵母 中图分类号：Q939.97；文献标识码：A ；文章篇号:1673-9078(2009)10-1107-08 Screening of Multiple-stress Resistant Saccharomyces Cerevisae by Genome Shuffling Methology 1,2 2 2 1 LIANG Lei , ZHANG Yuan-ping , HUANG Xiang-yang , LIANG Shi-zhong (1.School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006,China) (2.Guangzhou Sugarcane Industry Research Institute, Guangzhou 510316, China) Abstract: Using genome shuffling technology, the multiple stress resistant yeast was screened in this paper. 28 mutants were obtained by restructuring the genomes of three parent strains. It was found that MSR14 obtained from the second shuffling and MSR23 obtained from the third shuffling had the ability of multiple stress tolerance. With the two strains, the ethanol production reached 8.58%(v/v) and 8.86%(v/v) respectively under multiple stress, , which were higher by 7% and 10.5% respective than those of the best parent strain GZ-05. According to the 26SrDNA sequence analysis，both MSR14 and MSR23 were identified as Saccharomyces cerevisiae. Key words: ge