中国家驴的非洲起源研究.pdfVIP

HEREDITAS (Beijing) 2008 3 , 30(3): 324―328 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2008.00324 1 1 1 1 1, 3 2 1 卢长吉 , 谢文美 , 苏锐 , 葛庆兰 , 陈宏 , 沈善义 , 雷初朝 1. , 712100 2. , 251900 3. , 221116 : 对我国13 个家驴品种367 条序列(其中引用文献资料241 条) 的mtDNA D-loop 区399 bp 进行分析, 共检 测到96 种单倍型57 个多态位点, 其单倍型多样度为0.767~0.967, 核苷酸多样度为0.014~0.032, 表明我国家驴 的遗传多态性丰富。与3 个努比亚野驴、3 个索马里野驴和 6 个亚洲野驴的序列构建 NJ 系统发育树, 证明我 国家驴的母系起源为非洲野驴中的索马里驴和努比亚驴, 亚洲野驴不是中国家驴的母系祖先。 : 中国家驴; mtDNA D-loop; 遗传多样性; 起源 African origin of Chinese domestic donkeys 1 1 1 1 1, 3 2 LU Chang-Ji , XIE Wen-Mei , SU Rui , GE Qing-Lan , CHEN Hong , SHEN Shan-Yi , LEI Chu-Zhao1 1. College of Animal Science and Technology, Northwest A F University, Yangling 712100, China; 2. Shandong Tianlong Academy of Donkey, Wudi 251900, China; 3. Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou 221116, China Abstract: Analysis of the 367 mtDNA D-loop sequences (of which 241 sequences were collected from literature) of 399 bp in 13 Chinese domestic donkey breeds revealed 96 different haplotypes with 57 polymorphic sites. The haplotype diversity and the nucleotide diversity were 0.767−0.967 and 0.014−0.032, respectively, indicating abundant genetic diversity in Chi- nese domestic donkeys. The Neighbor-joining tree of Chinese domestic donkey sequences was constructed with 3 Nubian wild ass sequences, 3 Somali wild ass sequences and 6 Asian wild ass sequences. Our results suggest that the maternal an- cestor of Chinese domestic donkeys is highly likely to be Somali and Nubian of African wild ass instead of Asian wild ass.