454测序法在环境微生物生态研究中的应用.pdfVIP

生物技术通报 # # BIO TE CHN OLOG Y B ULLETIN 20 10 1 454 1 2 徐晓宇 刘和 1 2 ( , 214 122; , 2 14 122) : 传统的 Sanger测序技术虽已成熟, 但其速度和成本的限制满足不了大规模测序的要 第二代高通量测序技 术结合了乳胶微粒和皮升级反应的4 54 焦磷酸测序法, 作为一种高通量测序技术, 具有分析结果准确高速高灵敏度和高自 动化的特点对 4 54 测序法的技术原理和操作步骤进行了介绍, 对近年来运用该方法在环境微生物生态研究领域的进展进行 了综述 : 454 测序 环境微生物生态 宏基因组 Application of 454 Sequencing in Environm entalM icrobial Ecology 1 2 Xu X iaoyu L iu H e 1 2 ( School of M edicine and Pharmaceutical, Wux i 2 14122; School of Environment and CivilEngineering Jiangnan University, Wux i 214122) Abstrac:t T here has been a des ire to increase the throughput o DNA sequenc ing ev er since Sang er sequencing by cha in te rm ina- tion o r ragmentation techn iques coupled w ith e lectrophoretic size separa tion14 54 sequenc ing is the py rosequenc ing w hich comb ined w ith m icro lu id ics and p ico lite r reaction1 In th is article, the deve lopm ent o the 454 sequenc ing system w as discu ssed, and the app lication o 454 sequenc ing in env ironm enta lm icrob ia l eco logy w as rev iew ed1 K ey w ords: 454 sequenc ing Env ironm ental m icrob ia l ecology M etagenom ics Sanger 1977 , , 1 454 , , 111 焦磷酸测序法的原理 454 454 , 454 DNA ( emu lsion PCR , DNA system ) ( p ico liltre ) ( pyrophos- AT P [ 1] phate) ,