ISSR+analysis+of+Caragana+microphylla+(Leguminosae)+in+different+temperature+gradients.pdfVIP

的天空？ Sciences in Cold and Arid Regions Volume 7, Issue 1, February, 2015 Citation: Huang WD, Zhao XY, Li YL, et al., 2015. ISSR analysis of Caragana microphylla (Leguminosae) in different temperature gradients. Sciences in Cold and Arid Regions, 7(1): 0099–0103. DOI: 10.3724/SP.J.1226.2015.00099. ISSR analysis of Caragana microphylla (Leguminosae) in different temperature gradients * WenDa Huang , XueYong Zhao, YuLin Li, YuQiang Li, YaYong Luo, Jing Feng, Na Su Naiman Desertification Research Station, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, Gansu 730000, China *Correspondence to: Ph.D., WenDa Huang, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences. No. 320, West Donggang Road, Lanzhou, Gansu 730000, China. Tel: +86-931-4967203; E-mail: huangwenda2008@163.com Received: August 15, 2014 Accepted: November 21, 2014 ABSTRACT Caragana microphylla is the most dominant and constructive shrub species in the Horqin Sandy Land of northeastern China. We evaluated the level of genetic variation within and among C. microphylla populations sampled from three different temperature gradients in the Horqin Sandy Land by using inter-simple sequence repeat polymorphism (ISSR) molecular markers. The results show that eight ISSR primers generated 127 bands, of which 123 (96.85%) were polymorphic. At the species level, genetic diversity was relatively high (P = 96.85%, h = 0.3143, I = 0.4790). The highest gene