黄荆子乙酸乙酯提取物对人乳腺癌T47D细胞及其裸鼠移植瘤生长抑制的影响.pdfVIP

·432 · MOD ERN ONCOLOGY,M ar2009, VO I17 , NO 3 黄荆子乙酸乙酯提取物对人乳腺癌 T47D 细胞及其裸鼠移植瘤生长抑 制的影响 1 1 2 1 1 陈雪莲 ,姜 　浩 ,曹建国 , 尹 　婵 , 肖集文 Effec t of the a ce toa ce ta te extra c t of the Vitex N egundo Seeds on the g row th inh ib ition of hum an b rea st cancer T47D ce lls and xenogra fts in nude m ice 1 1 2 1 1 CH EN Xue - lian , J IAN G H ao , CAO J ian - guo , Y IN Chan , X IAO J i - wen 1 2 The F irs t Aff ilia ted H osp ita l of N anhua Un ivers ity, H engy ang 42 100 1, Ch ina; Cancer R esea rch Ins titu te of N anhua Un ivers ity , H engy ang 42 100 1, Ch ina. 【A b stract 】　O bjective: To inve stigate the effect of the acetoacetate extract of V itex N egu - ndo Seed s ( EV n - 50) on the grow th inh ib ition of hum an b rea st cancer T47D cells and xe - nografts in nude m ice. M ethod s:Hum an brea st cancer T47D cells were cu ltured in vitro. The nucleic acid synthe sis of EV n - 50 on T47D cells wa s exam ined by B r dU incorporating a ssay. Clone - form ation assay in p late wa s u sed to te st inh ib ition of clone form ation of T47D by EV n - 50. Hum an brea st cancer T47D xenografts in nude mou se model wa s esta - b lished to inve stigate the grow th inh ib i tion of EV n - 50. The morpho logy of tumor cells was ob served by m icro scopy after HE stain ing. Results: EV n - 50 in h ib ited nucleic acid synthe sis and grow th of T47D cells in a do se - dep enden t m anner; EV n - 50 inh ib ited the grow th of hum an b rea st cancer T47D xenografts in nude m ice, in a