大麦SSR标记遗传多样性及其与农艺性状关联分析_赖勇.pdfVIP

2013,46(2):233-242 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2013.02.002 1 2 /730070 730070 3 730070 Genetic Diversity and Association Analysis Using SSR Markers in Barley LAI Yong1,2, WANG Peng-xi1,2, FAN Gui-qiang1,2, SI Er-jing1,2, WANG Jin1,2, YANG Ke1,2, MENG Ya-xiong1,2, LI Bao-chun1,3, MA Xiao-le1,2, SHANG Xun-wu1,2, WANG Hua-jun1,2 1 ( Gansu Key Laboratory of Crop Improvement Germplasm Enhancement/Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou 730070; 2 3 College of Agronomy, Gansu Agricultural University, Lanzhou 730070; College of Life Sciences and Technology, Gansu Agricultural University, Lanzhou 730070) Abstract ObjectiveIn order to provide useful information for hybridization combination of parent materials and molecular assisted breeding, the genetic diversity of parent materials was determined by using SSR markers and their association with some agronomic traits were detected. Method A total of 86 SSR markers were screened for polymorphism among parent materials, and then the analysis on genetic diversity of these materials were detected. Population structure was analyzed through 57 selected markers, and then association analysis between SSR markers and 5 agronomic traits were performed using TASSEL GLM (General Linear Model) and MLM (Mixed Linear Model) programs.ResultA total of 200 alleles were found from 113 materials, ranged from 1 to 5. Allele frequency ranged from 0.00