鼠舌癌发生过程中弹性纤维胶原纤维的变化.pdfVIP

窑2696窑 南方医科大学学报(J South Med Univ) 2010;30(12) 鼠舌癌发生过程中弹性纤维尧胶原纤维的变化 1,2 1 1 1 1 1 1 1 张 鹏 袁杜玉宾 袁于 淼 袁殷 翔 袁吕岩红 袁髙钟秀子 袁郑金华 渊哈尔滨医科大学解剖学教研室袁黑龙江 哈 2 尔滨 150081曰齐齐哈尔医学院解剖教研室袁黑龙江 齐齐哈尔 161006冤 摘要院目的 观察金黄地鼠舌癌发生过程中弹性和胶原纤维的变化袁探讨细胞外基质代谢紊乱与癌进展关系的本质遥方 法 金黄地鼠舌癌存档蜡块袁分为对照渊6 只冤组和实验组渊42 只冤袁实验组已有明确的病理分级渊包括不典型增生尧原位 癌和早期浸润癌冤遥应用Masson 和醛品红染色检测各组中弹性和胶原纤维的构筑和分布遥结果 在对照组AF 染色阳性 的纤细弹性纤维构成结缔组织芯的框架袁在重度不典型增生和原位癌组弹性纤维呈现断裂和稀疏袁在早期浸润癌组弹 性纤维减少更为明显遥对照组的胶原纤维表达低于实验组袁在重度不典型增生和原位癌组胶原纤维显著增加袁排列紊 乱曰在早期浸润癌组胶原纤维不仅增粗并沉积于粘膜固有层遥 结论 癌的新生与鼠舌癌组织部位的胶原纤维过量沉积 和弹性纤维明显减少的变化是引起舌癌生长和进展的重要诱因遥 关键词院鼠舌癌曰胶原纤维曰弹性纤维曰 Masson 染色曰醛品红染色 中图分类号院R739.8 文献标志码院A 文章编号院1673-4254(2010)12-2696-03 Changesoftheelasticfibersandcollagenfibersduringthedevelopmentandprogressionof experimentallyinducedtonguecarcinomainhamsters 1,2 1 1 1 1 1 1 ZHANG Peng , DU Yu-bin , YU Miao , YIN Xiang , LV Yan-hong , GAO Zhong-xiu-zi , ZHENG Jin-hua 1 2 Department of Anatomy, Harbin Medical University, Harbin 150081, China; Department of Anatomy, Qiqihar Medical College, Qiqihar 161006, China Abstract:ObjectiveTo investigate the relationship between the extracellular matrix (ECM) and neoplastic progression in hamster with tongue cancer. MethodsForty-eight specimens of hamster tongue cancer were divided into control group (n=6) and experimental group (n=42). The pathological grade of the specimens was assessed (including 3 stages, namely atypical hyperplasia, carcinoma in situ and early invasive carcinoma). The sections of the tongue were stained with Masson and aldehyde-fuchsin (AF) staining for microscopic observation of the