Characterization+of+four+hemocyanin+isoforms+in+Litopenaeus+vannamei.pdfVIP

Acta Oceanol. Sin., 2015, Vol. 34, No. 2, P. 36–44 DOI: 10.1007/s13131-015-0588-9 E-mail: hyxbe@263.net Characterization of four hemocyanin isoforms in Litopenaeus vannamei 1, 2 2 5 4 3, 5 2 2 XU Jingxiang , RUAN Lingwei *, LI Zhen , YU Xiaoman , LI Sedong , SHI Hong , XU Xun 1 School of Life Science, Xiamen University, Xiamen 361005, China 2 Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China 3 Zhanjiang Ocean and Fishery Development Research Center, Zhanjiang 524000, China 4 School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China 5 Zhanjiang Evergreen South Ocean Science Technology Company Limited, Zhanjiang 524094, China Received 4 November 2013; accepted 27 February 2014 ©The Chinese Society of Oceanography and Springer-Verlag Berlin Heidelberg 2015 Abstract In this study, the gene encoding hemocyanin subunit L, LvHcL, was cloned from Litopenaeus vannamei and the genomic organization was characterized. This gene was diverse with many SNPs and also had at least four isoforms, while one of them (LvHcL4) only had two exons and the exon2 was missed. Transcription analysis showed that these isoforms of LvHcL were up -regulated after WSSV challenge in WSSV-resistant shrimp, while the transcriptions were decreased constantly in WSSV-susceptible shrimp. It is suggested that the hemocyanin had rich polymorphism and was involved in the antiviral response. These results could extend our previous findings and provide insights into the immune feature of hemocyanin, which would be helpful for further studies aimed at antiviral mechanism in inver- tebrate. Key words: hemocyanin, SNPs, isoforms, Litopenaeus vannamei, WSSV Citation: Xu Jingxiang, Ruan Lingwei, Li Zhen, Yu Xiaom