小麦矮秆种质系山农315041矮秆基因的鉴定及其染色体定位.pdfVIP

中国农业科学 2004,37(12):1942-1945 Scientia Agricultura Sinica 小麦矮秆种质系山农31504-1矮秆基因的鉴定 及其染色体定位 于东海 李 静 王洪刚 李兴峰 陆文辉 山东农业大学农学院/ 国家小麦改良中心山东分中心 泰安 271018 摘要 以小麦矮秆种质系山农31504-1 作母本与小麦高秆品种杂交 获得F 1 杂种和F2 分离群体 初步遗传分 析表明 山农31504-1 的矮秆性状由部分显性单基因控制 苗期外施赤霉酸表明 山农31504-1 对赤霉酸不敏感 同时利用中国春缺体- 四体系和中国春端体系对山农31504-1 矮秆基因进行了染色体定位 结果证明矮秆基因位于 山农31504-1 的2A 染色体上 关键词 小麦矮秆种质系 矮秆基因 染色体定位 Identification and Chromosome Location of Dwarf Gene in Short Stalked Germplasm Line Shannong31504-1 YU Dong-hai, LI Jing, WANG Hong-gang, LI Xing-feng, LU Wen-hui ( Agronomy College of Shandong Agricultu ral University/Shandong (Tai’an) Subcenter of National Wheat Improvement Center, Taian 271018) Abstract: Crosses between dwarf germplasm line Shannong31504-1, high material Shannong298 and Chinese Spring were made respectively. Through genetic analysis, it can be proved that the dwarf trait of Shannong31504-1 was controlled by one partial dominant gene. The GA-insensitivity of Shannong31504- 1was detected by treating seedlings with low concentrations of exogenous GA. In order to locate the dwarf gene in Shannong31504- 1, Chinese Spring nullitetrasomic lines and ditelosomic lines were crossed with Shannong31504-1. The result indicated that the dwarf gene was located on the chromosome 2A of Shannong31504-1. Key words: Wheat dwarf germplasm line; Dwarf gene; Chromosome location 20世纪后半叶 小麦矮化育种取得了巨大成就 体丢失1条时 表现为半矮秆 当附加的偃麦草染色 [1] 到目前为止 在小麦中共发现了21个矮秆基因 它 体全部丢失后 则表现为高秆 从而证明矮秆性状由 [3] 们来自普通小麦和硬粒小麦 其中Rht3Rht5Rht10附加的长穗偃麦草染色体控制 从山农 31504中选 Rht12Rht14Rht16Rht18Rht19