环氧化酶2和nm23H1蛋白表达及其与胆管癌的相关性.docVIP

环氧化酶-2和nm23H1蛋白表达及其与胆管癌的相关性 环氧化酶-2和nm23H1蛋白表达及其与胆管癌的相关性 作者：谷化平 周翠玲 作者单位：中国人民解放军第251医院病理科，河北 张家口 075000 【摘要】 目的 探讨环氧化酶-2（cyclooxygenase-2，COX-2）和nm23H1蛋白在胆管癌中的表达及临床意义。方法 应用免疫组化方法检测46例胆管癌和10例正常胆管组织中COX-2及nm23H1蛋白表达水平，并结合临床病理特征进行分析。结果 在胆管癌中COX-2及nm23H1蛋白阳性表达率分别为78.3%（32/46）和54.3%（25/46）。胆管癌中COX-2表达阳性率显著高于正常胆管组织(P0.05)，而nm23H1表达阳性率显著低于正常胆管组织(P0.05)。COX-2表达与胆管癌的TNM分期和转移相关（P0.05），而与胆管癌分化程度无关（P0.05）。nm23H1表达与胆管癌的TNM分期、分化程度和转移相关（P0.05）。结论 检测COX-2和nm23H1基因蛋白表达可作为评估胆管癌生物学行为和预后的参考指标。 【关键词】 胆管肿瘤 环氧化酶-2 nm23H1 基因表达调控 免疫组织化学 Expression of COX-2 and nm23H1 protein and its correlation with cholangiocarcinomas GU Huaping*， ZHOU Cuiling. * Department of Pathology, the 251st Hospital of PLA， Zhangjiakou 075000 Abstract Objective To investigate the expression of cyclooxygenase-2 （COX-2） and nm23H1 protein in cholangiocarcinomas (ChC) and its clinical significance. Methods Immunohistochemical method was used to investigate the expression of COX-2 and nm23H1 protein in 46 cases of ChC and 10 cases of normal choledochus(NCC). The relationship between expression of COX-2， nm23H1 and the clinicopathological factors of ChC was analyzed. Results The positive rate of COX-2 and nm23H was 78.3% （32/46） and 54.3% （25/46） respectively in ChC. The positive rate of COX-2 in ChC was higher than that in NCC (P0.05)， but the nm23H was lower than that in NCC （P0.05）. The expression of the COX-2 was positively correlated with the TNM phase and metastasis in ChC (P0.05)， but the expression of the COX-2 was no correlated with the differentiation degree in ChC(P0.05). The expression of nm23H1 was positively correlated with the TNM phase， differentiation degree and metastasis in ChC(P0.05). Conclusion Expression of tumor suppressor COX-2 and nm23H gene protein can be helpful in predicting the biological behavior and prognosis of ChC. Key words bile duct neoplasms； cyclooxygenase-2； nm23H1； gene expression regulation； immunohistochemistry 和L