细胞凋亡过程中Bid蛋白在活细胞内的动态分布.pdfVIP

14 4 Vol. 14 No. 4 2005 8 ACTA LASER BIOLOGY SINICA A ug . 2005 ## X Bid * XX , , , ( , 510631) : ( tumor necrosis factor-A, TNF-A) , Caspase8 Bid , C- c , Bid , Bid c Bid , , Bid : Bid : Q- 331 Q254 : A : 1007- 7146 ( 2005) 04- 0279- 04 Dynamics of Distribution of Bid Protein during TNF- A-induced Apoptosis in Living Cell * CH EN Tong-sheng , WANG Jin-j un , XING Da , WANG Fang ( Institute of Laser Life Science, South China Normal University, Gu angzhou 510631, Gu angdong, China) Abstract: When hum an lung cancer cells are exposed to death- inducing molecules su ch as tumor necrosis f actor-A, TNF-A) , caspase8 is activated and cleaves Bid into tw o fragments, then the C-terminal fragment relocates from cytosol to mitochondria and promotes the release of cytochrom e c, finally cellular apoptosis is indu ced. Desp ite recent progress in the stu dy of Bid during apop- tosis induction, it rem ains unclear how C-terminal fragm ent of Bid cleaved moves to mitochondria and then induces the release of cytochrome c and other substances . In order to further study the biological action of Bid during apoptosis, especially under physio- logical condition of living cell , the distribution of Bid in individual living cell during the apoptosis w as primarily inve