肠内免疫营养对创伤后大鼠肠屏障功能的影响.docVIP

肠内免疫营养对创伤后大鼠肠屏障功能的影响 　　作者：余利坚　徐亮　姚晖　魏仁志　綦晓龙　　作者单位：1泸州市人民医院　肛肠科　(四川　泸州　646000)　　2泸州医学院附属医院　胃肠外科　 (四川　泸州　646000) 　　【摘要】目的研究肠内免疫营养对创伤后大鼠肠道屏障功能的影响。方法: 将30只Wistar大鼠随机分为3组，即对照组、普通肠内营养组、肠内免疫营养组。行胃造瘘术后早期分别给予普通饲料、肠内营养剂、肠内免疫营养剂等，行肠内营养7 d，测量小肠黏膜形态和小肠黏膜IgA+、CD3+、CD4+和CD8+细胞数量。结果：小肠绒毛高度、肠腺隐窝深度、黏膜厚度以及绒毛表面积等相关的形态学参数测量值显示，肠内免疫组均优于对照组(P0.05)，与普通营养组相比差异无统计学意义 (P0.05)。对照组和普通肠内营养组大鼠小肠黏膜IgA+、CD3+、CD4+和CD8+细胞数低于肠内免疫营养组 (P0.05)。结论：肠内免疫营养能较好的改善术后大鼠的小肠机械屏障功能，促进小肠黏膜屏障功能的恢复，增强其肠道免疫功能。 　　【关键词】 肠道营养o肠内免疫营养o大鼠，Wistar 　　Effects of enteral immunonutrition on intestinal mucosal barrieral function of wound rats 　　YU Li-jian1, XU Liang2, YAO Hui2, WEI Ren-zhi2, QI Xiao-long2 　　1Department of General Surgery, Luzhou Peoples Hospital (Luzhou 646000 , China) 　　2Department of Gastrointestianal Surgery, The Affiliated Hospital of Luzhou Medical College(Luzhou 646000, China) 　　【ABSTRACT】 Objective: To study effects of enteral immunonutrition on intestinal mucosa barrieral function in wound rats. Methods: 30 Wistar rats were randomly divided into three groups ,with ten rats in each group(ie.control group,enteral nutrition and enteral immunonutrition group). After the germ-free operation of gastrostomy, rats in each group of enteral nutrition were treated with thesocaloric and isonitrogenous nutritional formulas respectively for 7 days.The ileum membrane morphological parameters and the quantity of IgA+,CD3+,CD4+ and CD8+ cell (each HP) in the ileum membrane were detected.Results: The villous height, crypt depth, mucosal thickness and villous surface area of ileum were increased in EN and EIN compared with control group(P0.05),but it is no significance between them(P0.05). The quantity of IgA+,CD3+,CD4+ and CD8+ cell is increased in EIN compared with control and EN group. (P0.05). Conclusion: enteral immunonutrition may improve intestine mechanical barrierl function , promote restoration of the rat small intestine mucous membrane barrier function, and improve intestine immune barr