膀胱肿瘤细胞多药耐药检测在浸润性膀胱肿瘤治疗中的应用.docVIP

膀胱肿瘤细胞多药耐药检测在浸润性膀胱肿瘤治疗中的应用 　　作者：吴小鹏，陆曙炎，蔡雷铭，邹建纲，周忠兴，关乃富，陈仰之，陈静　(南京医科大学附属常州第二医院泌尿外科，江苏常州 213003) 　　Effect of histamine H3 receptors in nucleus tractus solitarius on c-Fos expression in the brain of rats with asthma onset 　　HU Chun-mei，DONG Rong，LIU Bao-sen，ZHANG Xiao-hong，JI Ji 　　(1.The6th Ward，Nanjing Thoracic Hospital，Nanjing210009，China;2.Department of Physiology and Pharmacology，School of Basic Medical Science，Southeast University，Nanjing210009，China) 　　Abstract:Objective To explore the effect of histamine H3 receptors in nucleus tractus solitarius(NTS)on c-Fos expres-sion in the brain of rats with asthma onset.Methods Male Sprague-Dawley(SD)rats were sensitized with ovalbumin to in-duce asthma using central stereotaxic and microinjection techniques.The model rats were randomly divided into three groups，injected NS，R-Α-methylhistamine(RMHA，a H3 receptor receptors agonist)，and thioperamide(Thio，a H3 receptors antago-nist)+RMHA into NTS respectively.Fos positive cells distributed in different nucleus were detected by immunohistochemistry SABC method after the challenge one hour later.Results After microinjection1Μg RMHA into NTS at the onset of asthma，the number of Fos positive cell and Fos density presented in NTS，periventricular hypothalamic nucleus(Pe)，hypothalamic paraventricular nucleus(Pa)，and raraventricular thalamic nucleus(PVN)decreased markedly(P0.01).These effects can be blocked by pre-treatmentwith5Μg microinjection of Thiop into NTS.Conclusion This study suggests that histamine H3 re-ceptors in NTS can ameliorate asthma via regulating some neurons in Pa，Pe and PVN. 　　Key words:rats;nucleus tractus solitarius;histamine H3 receptors;asthma;Fos protein;expression 　　[摘要]目的: 检测膀胱肿瘤细胞多药耐药情况，以选择敏感药物治疗浸润性膀胱肿瘤。 方法: 双侧髂内动脉灌注化疗前采用免疫组织化学方法对10例膀胱肿瘤病例作多药耐药糖蛋白(P.gp)、谷胱甘肽S转移酶(GST.p)和拓扑异构酶Ⅱ(TopoⅡ)表达情况检测。 结果: 灌注前P.gp的表达率为30%，GST.p为10%，TopoⅡ为50%。灌注药物选择吡柔比星、顺铂和羟基喜树碱。灌注后P.gp的表达率为50%，GST.p为10%，TopoⅡ为40%。 结论: 检测P.gp、GST.p、TopoⅡ表达情况对浸润性膀胱肿瘤化疗药物的选择具有重要