菠菜rDNA及端粒多色荧光原位杂交分析.pdfVIP

HEREDITAS (Beijing) 2007 11 , 29(11): 1405―1408 ISSN 0253-9772 研究报告 DOI: 10.1360/yc-007-1405 rDNA 兰添颖, 刘博, 董凤平, 陈瑞阳, 李秀兰, 陈成彬 , 300071 : 以拟南芥25S rDNA 、5S rDNA 以及端粒序列为探针对菠菜的中期染色体进行了多色荧光原位杂交分析, 其中25S rDNA 用生物素标记, 端粒序列用地高辛标记, 5S rDNA 用生物素和地高辛共同标记。杂交结果显示, 菠菜中期染色体25S rDNA 杂交位点为6 个, 分别位于3 号、5 号和6 号染色体的随体部位; 5S rDNA 杂交位点 为4 个, 分别位于3 号和5 号染色体长臂, 端粒序列杂交位点位于6 号染色体端部以及其余染色体的端部和着 丝粒部位。 : 菠菜; 多色荧光原位杂交; rDNA; 端粒 Multicolor FISH analysis of rDNA and telomere on spin- ach LAN Tian-Ying, LIU Bo, DONG Feng-Ping, CHEN Rui-Yang, LI Xiu-Lan, CHEN Cheng-Bin College of Life Sciences, Nankai University, Tianjin 300071, China Abstract: In this study, multicolor FISH analysis on metaphase chromosomes of spinach with biotin-labeled 25S rDNA, DIG-labeled telomere sequences and biotin-labeled and DIG-labeled 5S rDNA was performed. There were six 25S rDNA loci, which were located on the satellites of the third, the fifth and the sixth chromosomes, four 5S rDNA loci, which were located on the long arms of the third and the fifth chromosomes. The telomere loci were located on the end of the sixth chromosome and also on both the end and centromeric regions of other chromosomes. This study is an important comple- ment to both traditional karyotype analysis and FISH karyotype analysis in spinach. Keywords: spinach; multicolor FISH; rDNA; telomere , (Silene latifolia)[1,2]RNA , (rDNA) , (FISH), , [3] , rDNA FISH , , , , , DNA, 3 收稿日期: 2007−04−16; 修回日期: 2007−05−29 作者简介: (1982−), , , , E-m