薄层色谱法分离菠菜叶片中色素的研究.pdfVIP

23 2 Vol.23 No.2 2000 4 Journal of Agricultural University of Hebei Apr.2000 : 1000- 1573(2000) 02- 0101- 04 X 果秀敏, 翟彤宇, 牛延锋 ( , 071001) : 菠菜叶片中富含多种色素成分, 为了对其进行分离鉴定, 用薄层色谱法着重研究了提取液展开剂等因 素对色素分离的影响, 选择了石油醚(60~ 90e ) - 无水乙醇( 3B2, V/ V) 的混合溶液作提取液, 石油醚( 60~ 90e ) - 丙酮- 苯( 2B 1. 5B2, V/ V) 的混合溶液作展开剂的最佳条件, 单向 一次性层析得到的7 个斑点清晰集 中, 简便快捷, 性能优异层析后结合薄层扫描对其结果进行了鉴定分析, 取得了满意的效果 : 薄层色谱; 叶绿素; 类胡萝卜素; 菠菜; 薄层扫描 : O 65216 : A Study of seperating pigments from spinacia using thin layer chromatography GUO Xiu- min, ZHAI Tong-yu, NIU Yan-feng (Department of asic Courses, Agricltural University of Hebei, aoding 071001,China) Abstract : The spinacia is rich in a variety of pigments. In order to separate and identify it, thin lay- er chromatography was used to study how such factors as extractant, developping agent, etc influ- ence the pigments seperating. T he experiment selected the mixture solution of petroleum ether( 60~ 90e ) and alcohol( 3B2, V/ V) as estractant, and the mixture solution of petroleum ether (60~ 90e ) and acetone and benzene(2B1.5B2,V/ V) as developping agent, utilized the best conditions of these solutions. The seperating result wasseven spots. They were centralized and clear. The exper-i ment was easy and quick, and the result was of high quality. After seperating, the result was ident-i fied and analysed by thin- layer scanning. It was proved that the results were satisfactory. Key words: thin- layer chromatography; chlorophyl; carotenoids; spinacia; thin- layer scanning , [ 1~ 3] , , , ,, (10~ 15 min) , 1