辛伐他汀阿托伐他汀对脑缺血再灌注大鼠血清MDASOD的影响.docVIP

辛伐他汀、阿托伐他汀对脑缺血再灌注大鼠血清MDA、SOD的影响 　　作者：袁胜山　余昌胤，张骏，蔡志友，陈然 作者单位：(1.右江民族医学院附属医院神经内科，广西百色533000;2.遵义医学院附属医院神经内科，贵州遵义563003;3.安徽省六安市人民医院神经内科，安徽六安237005) 　　【摘要】 目的 观察阿托伐他汀、辛伐他汀对大鼠脑缺血再灌注血清超氧化物歧化酶(SOD)和丙二醛(MDA)浓度的影响，探讨他汀类药物对脑缺血再灌注后自由基的清除作用。方法 将36只Wistar大鼠随机分为假手术组，缺血再灌注组，阿托伐他汀预处理高、低剂量组，辛伐他汀预处理高、低剂量组(每组6只)。高、低剂量组分别予6mg/(kg·d)和1.5mg/(kg·d)阿托伐他汀/辛伐他汀灌胃，连续给药7天。用线栓法制备大脑中动脉缺血再灌注模型，缺血2h，再灌注22h。用比色法检测外周血中MDA、SOD的浓度。HE染色观察大鼠脑组织的病理学改变。结果 辛伐他汀、阿托伐他汀预处理各组外周血SOD浓度较缺血再灌注组显著升高、MDA浓度显著降低(P0.05或P0.01)。各预处理组之间差异无显著性(P0.05)。结论 辛伐他汀、阿托伐他汀预处理能提高大鼠脑缺血再灌注后外周血SOD浓度、降低MDA浓度。 　　【关键词】 脑缺血;再灌注损伤;辛伐他汀;阿托伐他汀;超氧化物歧化酶;丙二醛 　　Simvastatin and atorvastatin influence on serum MDA, SODin rats with focal cerebral ischemia-reperfusion injury 　　YUAN Sheng-shan1, YU Chang-yin2, Zhang Jun2, CAI Zhi-you2, CHEN Ran3 　　(1. Department of Neurology, Affiliated Hospital of Youjiang Medical College for Nationalities, 　　Baise, Guangxi 533000, China; 2. Department of Neurology, Affiliated Hospital of Zunyi Medical 　　College, Zunyi, Guizhou 563003, China; 3. Department of Neurology, Liuan Peoples 　　Hospital, Liuan, Anhui 237005, China) 　　Abstract: Objective To observe the influences of simvastatin (Sim) and atorvastatin (Ator) on concentration of serum malondialdehyde (MDA), superoxide dismutase (SOD) in rats with focal cerebral ischemia reperfusion injury and to evaluate the capability of tatin drugs for free radical scavenging. Methods 36 Wistar rats were randomly divided into: sham ischemic group (Sham group), ischemia-reperfusion group (IR group), and Sim and Ator preconditioning in high and low dose groups, 6 rats in each group. High and low dose groups were respectively oral perfused with 6mg/(kg·d) and 1.5 mg/(kg·d) for 7 days continuously. To produce the focal cerebral ischemia-reperfusion injury model, the middle cerebral artery of rat was occluded by inserting a nylon suture through the internal carotid artery for 2h, followed by reperfusion for 22h after withdrawing the suture. The