非小细胞肺癌病人外周血MUC1基因定量表达及意义.docVIP

非小细胞肺癌病人外周血MUC1基因定量表达及意义 　　作者：金翔凤,罗宜人,王明钊,徐晓娟，沈毅,张永红 作者单位：青岛大学医学院附属医院胸外科,山东 青岛 266003;日本山口大学医学部; 青岛大学医学院附属医院神经内科;[基金项目]青岛市科技局科研基金项目 (031NY142) 　　【摘要】 　　目的 探讨Mucin1(MUC1) mRNA在非小细胞肺癌(NSCLC)病人外周血中的表达及临床意义。方法 采用实时荧光定量RTPCR方法检测MUC1 mRNA在NSCLC病人外周血中的表达。结果 NSCLC病人外周血中MUC1 mRNA表达显著高于正常人(t=2.01，P0.05);鳞癌与腺癌病人MUC1基因的表达无明显差异(P0.05)。NSCLC病人外周血中MUC1 mRNA表达与NSCLC的病理分级、TNM分期及淋巴结转移密切相关(F=25.62、34.61，t=2.05，P0.05)。结论 NSCLC病人外周血中MUC1 mRNA表达与NSCLC发生、发展、侵袭、转移及恶性程度密切相关，对判断NSCLC恶性程度及预后有重要参考价值。 　　【关键词】 CA153抗原　癌，非小细胞肺　逆转录聚合酶链反应 　　QUANTITATIVE EXPRESSION OF MUC1 mRNA IN PERIPHERAL BLOOD AND ITS CLINICAL SIGNIFICANCE IN PATIENTS WITH NONSMALL CELL LUNG CANCER 　　JIN XIANGFENG, LUO YIREN, WANG MINGZHAO, et al 　　(Department of Thoracic Surgey, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China); 　　[ABSTRACT] Objective To explore the expression and clinical significance of MUC1 mRNA in peripheral blood of patients with nonsmall cell lung cancer (NSCLC). Methods The expression of MUC1 mRNA in peripheral blood of NSCLC patients was detected by realtime fluorescent quantitative RTPCR. Results The expression of MUC1 mRNA in the patients was singnificantly higher than that of healthy controls (t=2.01,P0.05). No significant difference of MUC1 mRNA expression was noted between the patients with squamous carcinoma and adencarcinoma (P0.05). Levels of MUC1 mRNA were closely correlated with pathological grade,TNM stage and lymphatic metastasis (F=25.62,34.61;t=2.05;P0.05). Conclusion Levels of MUC1 mRNA expression in patients with NSCLC closely correlates with the occurance, development, invasion, metastasis and extent of malignancy of the disease, which are of a great reference in estimating the malignancy and prognosis of NSCLC. 　　[KEY WORDS] CA153 antigen; cancer, nonsmallcell lung; reverse transcriptase polymerase chain reaction 　　肺癌是呼吸系统常见的恶性肿瘤，目前已成为国内外发病率和死亡率均位居首位的的恶性肿瘤，其中非小细胞肺癌(NSCLC)的术后5年生存率仅30%左右。即使是完全切除的Ⅰ期NSC