早期胃癌组织中VEGF-C表达与淋巴结微转移关系的研究 Expression of vascular endothelial growth factor-C and its correlation with lymph node micrometastasis in early gastric carcinoma.pdfVIP

江医学2012年第34 卷第3 期 早期胃癌组织中VEGF-C 表达与 淋巴结微转移关系的研究 胡逸人 郑志强 【摘要 】 目的 研究早期胃癌组织中血管内皮生长因子-C（VEGF-C ）表达水平及其与淋巴结微转移的关系。 方法 采用免 疫组化技术检测48 例常规HE 染色无淋巴结转移的早期胃癌患者胃癌组织中VEGF-C 的表达水平，采用淋巴结组织HE 染色和细胞 角蛋白（CK ）20 表达检测判断淋巴结微转移情况，分析VEGF-C 表达水平与淋巴结微转移的关系。 结果 48 例患者中，7 例患者的 14 枚淋巴结发现CK20 阳性细胞，阳性率 14.6％（7/48 ），淋巴结微转移率3.6％( 14/385) ；有淋巴结微转移组VEGF-C 阳性表达率 85.7％（6/7 ），明显高于无淋巴结微转移组的31.7％（13/41 ），差异有统计学意义（P 0.05 ）。 结论 检测CK20 的表达了解淋巴结 微转移较传 病理检查敏感，适用于微转移诊断；胃癌组织VEGF-C 高表达有较高的淋巴结微转移风险，这对判断预后和指导治疗 有重要意义。 【关键词】 血管内皮生长因子-C 细胞角蛋白20 淋巴结微转移 早期胃癌 Expression of vascular endothelial growth factor-C and its correlation with lymph node micrometastasis in early gastric carcinoma China 【Abstract 】 Obj ective To investigate the expression of vascular endothelial growth factor C (VEGF-C) in early gastric carcinoma and its correlation with micrometastasis of the lymph nodes. Methods Immunohistochemistry (IHC) was used to de- tect the VEGF-C expression in 48 cases of early gastric carcinoma, in which lymph node metastases was not detected by con- ventional hematoxylin-eosin (HE)staining. HE staining and cytokeratin (CK)20 were used to examine the micrometastasis of the lymph nodes. The relationship between expression of VEGF-C and the micrometastasis of lymph nodes was analyzed. Results Lymph node micrometastasis was identified in 7 out of 48 patients (14.6％) and 14 out of 385 nodes (3.6％)．The positive ex- pression of VEGF-C was detected in 6 (85 .7％) of 7 patients with lymph node micrometastases ，and in 13(3 1.7％)of 41 patients without lymph node micrometastases．The level of VEGF-C expression in lymph node micrometastases was significantly higher than that in patients without lymph node micrometastases. The difference between the two groups was statistically significant (P 0.05). Conclusion High expression of VEGF-C in ga