《A Modified Method for the Development of SSR Molecular Markers Based on Redundant EST Data and Its Application in Soybean》.pdfVIP
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Availableonlineat磷ww.sciencedimct.com
。~≯“8
JournalofIntegrativeAgriculture 。a∥3嚣嚣” ScienceDirect
2012
2012,11(4j:545—555 April
AModifiedMethodforthe ofSSRMolecularMarkersBas
Development
ESTDataandIts in
onRedundant ApplicationSoybean
ZHAO Wei—liandLIWen-bin
Xue+,CHANGWei+,HAN
Ying—peng,TENG
ResearchInstitute,Northeast 150030
KeyLaboratoryofSol,beanBiology.Ministry AgriculturalUniversity,Harbin
ofEducation/Soybean
Jp月C矗I”口
AbstracC
EST-derivedSSRmarkerhasbeen in fewmethodsof havebeen for
developedmanyspecies,but highefficiency reported
datais
the ofEST-SSR methodfor millionsofredundantEST needed
exploitation markers.Thus,ahighefficiency mining
AmodifiedmethodfortheEST-SSR with wasestablishedbasedontheredundantESTdata
developmenthighefficiency
of inthis methodachieveditsfunction ESTs tothesameSSRmotifand
soybeanstudy.The throughclassifyingaccording
lociwithredundant this totalof80 EST-SSRmarkersof
detectedcandidate sequences.Instudy,a polymorphic soybean
were ofthemwere thismodifiedmethodwhich the and ofthis
developed,50 exploitedby provedhigherspeedefficiency
wer
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