卡维地洛抗动脉粥样硬化作用研究.docVIP

卡维地洛抗动脉粥样硬化作用的研究 　　作者：田建会 作者单位：青岛大学医学院附属医院心内科，山东 青岛 266003 　　【摘要】 目的 探讨卡维地洛在动脉粥样硬化病变中的抗氧化作用及其对增殖细胞核抗原(PCNA)及核因子kappaBp65(NFΚBp65)活化的影响。方法 雄性新西兰大耳白兔24只，随机分为正常组、高脂组、卡维地洛组，每组8只，共计喂养10周。实验结束后，酶法检测各组家兔的血清三酰甘油及胆固醇，黄嘌呤氧化法检测血清超氧化物歧化酶(SOD)，硫代巴比妥酸比色法检测丙二醛(MDA),并采用免疫组化的方法测定血管壁PCNA及NFΚBp65的表达。结果 高脂组和卡维地洛组血清三酰甘油和胆固醇较正常组明显升高(F=146.25、287.83,q=14.76～20.75,P0.01)，而该两组间差别无统计学意义(q=0.53、0.59,P0.05)。高脂组较正常组SOD明显降低，MDA明显增加，而卡维地洛组较高脂组SOD有所升高，MDA有所降低(F=21.65、75.64,q=4.98～24.29,P0.01)。高脂组PCNA和NFΚBp65的表达较正常组明显增加，卡维地洛组PCNA和NFΚBp65的表达较高脂组明显减少(F=426.12、521.38,q=56.15～75.87,P0.01)。PCNA和NFΚBp65的呈正相关(r=0.975，P0.01)。结论 卡维地洛有减轻脂质过氧化损伤的作用，可阻断NFΚB的活化，进而抑制平滑肌细胞的增殖。 　　【关键词】 动脉硬化 卡维地洛 抗氧化剂 细胞增殖 兔 　　AN EXPERIMENTAL STUDY OF THE EFFECT OF CARVEDILOL ON ATHEROSCLEROSISTIAN JIANHUI, DING SIHUA, QI PING, et al (Department of Cardiology, The Affiliated Hospital of Qingdao University Medical College， Qingdao 266003, China) [ABSTRACT]ObjectiveTo study the effect of carvedilol on antioxidation and activation of PCAN and NFΚB on atherosclerosis.MethodsTwentyfour male New Zealand white rabbits were randomly divided into three groups: normal group, highfat diet group and cavediloltreated group, with eight rabbits in each group. All the rabbits were fed for 10 weeks. After completion of the experiment, the levels of serum triglyceride and cholesterol were measured by enzymatic method, of serum SOD by xanthine oxidative technique, and of serum MDA by thiobarbituric acid method. Expression of the PCAN and NFΚBp65 in the vascular wall was detected immunohistochemically. ResultsCompared with those of normal rabbits, the levels of serum triglyceride and cholesterol of the highfat diet and cavediloltreated were extremely high (F=146.25，287.83; q=14.76-20.75; P0.01), but no statistical difference was found between the latter two groups (q=0.53，0.59; P0.05). Lowlevel serum SOD and highlevel serum MDA were noticed in highfat diet rabbits. In highfat diet group, SOD was lower than that in normal group