乌药叶中总黄酮的含量测定.pdfVIP

39 9 Vo.l 39 No. 9 2010 9 Applied Chem ical Industry Sep. 2010 1 2 1 许海丹, 陈珍 , 顾霞敏 ( 1. , 317000; 2. , 317000) : A lC l , , 3 A lC l , 4 15 nm 3 2 , 0. 002 ~ 0. 020 m g/mL, , A = 30. 076C + 0. 006 5, R = 0. 999 6, = 101. 0% , RSD= 1. 27% ( n= 6) : ; ; A lC l ; 3 : O 657. 32 : A : 1671- 3206(2010) 09- 1405- 03 Determination of total flavonoids in Lindera aggregata leaves 1 2 1 X UH a idan , CH EN Zh en , G UX iam in ( 1. Departm ent of Pharm aceutical and Chem ical Engineering, Taizhou Un iversity, L inhai 317000, Ch ina; 2. School of L ife Science, Taizhou Un iversity, L inhai 317000, Ch ina) Abstract: A spectrophotom etric method for the determ ination of total flavonoids extracted from the leaves of L indera aggregate ( Smi s) K osterm. w as established. In the solution of subacidity, the total flavonoids in L indera aggregate leaves reactsw ith A lCl to form a stable yellow complex, itsmaxmi um absorption wave 3 2 length is 415 nm. The regression equation is A = 30. 076C + 0. 006 5, correlation coefficient R is 0. 999 6. The absorbance w as linear w ith concentration of the total flavonoids in the range of 0. 002 ~ 0. 020 mg /mL. The average recovery is 101. 0% ( RSD = 1. 27% , n= 6). This method is smi ple, rap