小麦叶片衰老过程中CAT和APX活力及其同工酶谱的变化X.pdf

　 J ou rna l of N anj ing A g ricu ltu ra l U n iversity 　 小麦叶片衰老过程中CAT 和APX 活力及其同工酶谱的变化 冯　晴　徐朗莱　叶茂炳　沈文飚 (南京农业大学理学院, 南京 210095) 摘要　小麦叶片在连体自然衰老和离体暗诱导衰老过程中, 过氧化氢酶(CAT) 活力比抗坏血酸过氧化物酶 (APX) 活力下降得早。当CAT 活力处于较低水平时, APX 仍然维持较高活力, 且APX 活力与 H2O 2 含量呈显 著负相关。经同工酶电泳分析, 离体叶片暗诱导衰老过程中, CAT 同工酶带无增减, 只是活性改变, 而APX 同 工酶带则有增加。APX 在小麦叶片衰老的中后期可能起着比CAT 更重要的清除 H O 的作用。 2 2 关键词　小麦; 叶片; 衰老; H O ; 同工酶; 过氧化氢酶; 抗坏血酸过氧化物酶 2 2 中图分类号　 512101 S CHANGES OF ACTIV ITIES AND ISOENZYM IC ZYMOGRAM OF CAT AND APX D UR ING SENESCENCE OF W HEAT L EAVES Feng Q ing, Xu L anglai, Ye M aob ing and Shen W enb iao (Co llege of Sciences, N an jing A gric U n iv , N an jing 210095) 　 ( ) ( ) ABSTRACT T he activity of asco rbate peroxidase A PX decreased later than that of catalase CA T during . , the course of senescence of intact and detached w heat leaves W hen the activity level of CA T w as low er that of A PX still rem ained h igher. T he A PX activity w as negatively co rrelated w ith the content of hydrogen per . , , oxide Furthermo re tw o additional bands w ere found on the isozymogram of A PX w h ile the band num ber of