白芨粗多糖提取方法的比较研究.pdfVIP

14 201 1 1 2 1 1 1 1 芮海云 吴国荣 张卫明 陆长梅 顾龚平 宰学明 邵志广 ( 1. 210097 2. 210042) 以白芨( Bletilla striata ( Thunb.) Reichb. f.) 块茎为原料, 运用多 理化方法, 提取白芨粗多糖, 经红 外吸收光谱紫外吸收光谱的比较和鉴定确认提取物中有多糖特征吸收测定粗多糖中总糖还原糖蛋白质等组 成物质的含量, 以多糖纯度和提取率为主要指标, 对提取方法予以比较结果表明热水处理和室温下超声处理为 较理想的提取方法 白芨; 多糖; 提取 Research on Extraction of polysaccharide from Bl etil la st rat a ( Thunb. ) Reichb. f. 1 1 2 1 1 1 1 Rui Haiyun , Wu Guorong , Zhang Weiming , u Changmei , Gu Gongping , Zai Xueming , Shao Zhiguang (1. College of Lif e Science, Nanj ing Normal University, Nanj ing 210097; 2. Nanj ing Academyf or the Comprehensive Utilization of ild Plants, Nanj ing 210042) Abstract Polysaccharides were extracted by several kinds of physical and chemical methods from Bletillastriata(Thunb.) Reichb. f. . By means of IR and UVspectrums, it was identified that there were polysaccharides in these extractions. Analyzing their total sugar, content of reducing sugar and protein, these methods were compared.According to the yield of extraction and purification rate, hot water and supersonic treatments are good methods for extraction of polysaccharide from Bletillastria ta(Thunb.) Reichb.f. Key words Bletillastriata(Thunb.) Reichb.f. , polysaccharide, extraction ( Bletilla striata(Thunb.) Reichb. f.) 1. 2 方法 1. 2. 1 多糖的提取 , , , , , 20g, , , 1. 2. 1. 1 不同提取剂回流提取 1. 5% [ 1] - 1 1 3% 0. 15mol NaOH 0. 3mol NaOH 20/ 1(W/ V) , 60 90 , 4 1 ,