热疗对肺癌A549细胞周期和凋亡的影响.docVIP

热疗对肺癌A549细胞周期和凋亡的影响 　　作者：蒋东，李 芳，沈文明，郑世营 作者单位：苏州大学附属第一医院胸心外科，苏州215006 　　【摘要】 目的：研究在不同温度、不同加热时间的条件下热疗对人肺腺癌A549细胞生长的影响。方法：不同温度、不同时间加热后，采用流式细胞技术观察热疗对人肺腺癌A549细胞株细胞周期和凋亡的影响。结果：流式细胞技术检测显示A549细胞经热疗处理后，细胞生长相应的滞留于细胞生长周期的G1期，细胞凋亡明显，细胞减少。热疗在不同温度、不同时间加热时间的条件下，导致A549细胞出现凋亡和坏死的程度不同，其中加热至42 ℃维持2 h处理组中凋亡最为明显。结论:热疗可抑制人肺腺癌A549细胞的增殖,使细胞周期阻滞于G1期,并且在加热至42 ℃维持2 h时细胞凋亡最为明显。 　　【关键词】 肺癌;热疗;细胞周期;凋亡 　　The influence of thermotherapy on the cell cycle and apoptosis of pulmonary carcinosis A549 cell 　　1JIANG Dong，2LI Fang，1SHEN Wenming，et al (1Department of Cardiothoracic Surgery, First Affiliated Hospital of Soochow University, Suzhou 215006; 2Department of Histology and Embryology, Medical School of Soochow University) 　　[Abstract] Objective: To investigate the influence of thermotherapy on the growth of pulmonary carcinosis A549 cell under different temperature and heating time. Methods: The flow cytometry was adopted to observe the influence on the growth of cells, cell cycle, and the apoptosis of pulmonary carcinosis A549 cell, after the cell was treated by thermotherapy under different temperature and heating time. Results: The flow cytometer shows that the cell cycle of A549 cell was arrested in G1 phrase, the apoptosis was obviously, and the number of A549 cell was decreased. The apoptosis and cell necrosis degree of A549 cell was various among the different conditions of thermotherapy and in the group heated for 2 hours under 42 ℃, remarkable apoptosis could be observed. Conclusion: The proliferation of pulmonary carcinosis A549 cell was inhibited, the cell cycle of A549 cell was arrested in G1 phrase by thermotherapy, and remarkable apoptosis could be observed when the A549 cell was heated for 2 hours under 42 ℃. 　　[Keywords] Pulmonary carcinoma; Thermotherapy; Cell cycle; Apoptosis 　　近年来肿瘤热疗已成为继手术、化疗、放疗、生物治疗之后的对肿瘤的第5类有效治疗手段，但其机制还不完全清楚。本实验以我们培养的肺癌A549细胞株为研究对象，观察不同温度、不同时间热疗对其细胞周期及凋亡的影响。流式细胞仪检测细胞周期及凋亡情况，以期对临床治疗提供有益的探索，并初步探讨其机制。 　　1 材料和方法 　　1.1 材料 人肺癌A5