桑树韧皮纤维生物脱胶菌种产果胶酶的分离及应用研究.pdf

2009 No．11 · · Serial No．212 China Brewing 86 Experience Exchanges 桑树韧皮纤维生物脱胶菌种产果胶酶的分离及应用研究 1 2 1 1 1 姜晓雷，慕 睿，何连芳*，赵长新，刘秉钺 （1.大连工业大学生物与食品工程学院，辽宁大连 116034；2.潍坊高新技术创业服务中心，山东 潍坊 261061） 摘 要：从沤麻水筛选出的No.9菌果胶酶经(NH ）SO 分级沉淀、透析，CM-Sephadex C-50离子交换柱、Sephadex G-100柱凝胶层析分 4 2 4 离纯化，分离出电泳纯的果胶裂解酶，测得分子量为43 kD ，最适反应温度为50℃，最适pH值为5.2；利用气相色谱法测得发酵液中果 胶酯酶酶活为7μg/mL。 关 键 词：桑树；纤维脱胶；果胶酶；分离纯化 中图分类号：Q556 文献标识码：B 文章编号：0254-5071 （2009）11-0086-03 Purification and properties of the pectinas form Mulberry Fiber degumming microbial strain 1 2 1 1 1 JIANG Xiaolei , MU Rui , HE Lianfang *, ZHAO Changxin , LIU Bingyue ( 1. College of Bio Food Tech, Dalian Polytechnic University, Dalian 116034, China; 2. Weifang High -Tech Innovation Service Center, Weifang 261061, China) Abstract: The pectinase from strain No.9 which was isolated from flax retting effluent was separated and purified by (NH ) SO sedimentation and 4 2 4 dialysis, CM-Sephadex C-50 gel anion exchange filtration and Sephadex G-100 gel filtration. The pectinase got electrophoretic purity, and its molec- ular weight is 43 kd. The temperature for optimal reaction was 50℃, the optimal pH was 5.2. Pectinesterase activity was 7 μg/ml measured by the gas chromatography Key words: mulberry ；fiber degumming；pectinase ；separated and purified 我国是桑树种植大国，种植面积800万亩，可年产干桑