自体骨髓间充质干细胞移植治疗脑创伤的实验研究.pdf

306 () 2009 11 3 4 Chin J Transplant ( ElectronicEdition), November 2009, ol 3, No. 4 刘猛 步星耀 程培训 张圣旭 鲍玉洲 ! (BM SC) Feeney DM SpragueDaw ley BM SC (NSS) Y, 5( BrdU)( GFAP) ( BDNF)nestin∀ , TUNEL , BM SC NSSY, , BrdU /GFAPBDNFnestin, (P 0. 05) BMSC, , , , ! ; ; Experim ental study on auto logous bonemarrow m esenchymal stem cell transplantation for traumatic * brain injury LIU M eng , BU X ingyao, CHEN G P eixun, ZHAN G Shengxu, BAO Yuzhou. * D e artm ent of N eurosurgery, H enan Provincial P eo le# sH os ita,l Zhengzhou 450003, China Corres ond ing author: B UX ingyao, Em ail: bux ingyao@ 126. com Abstract! Objective To explore the efficacy and mechanism of treating traumatic brain injury (TBI) in rats by surgical transplantation of autologous bone marrow mesenchymal stem cells (BMSC). M ethods After being given TBI by using DennisM. Feeney method, SpragueDawley ratswere random ly divided into the treated group inwhich autologous BM SCwere transplanted into the trauma area by surgi caloperation and the control group with saline. Function of the nervous system in rats was analyzed by Yelectric maze test and the neurological severity scores (NSS). H istopathology was observed and mi mu nohistochem istry was performed to detect bromodeoxyuridine ( BrdU ), glial fibrillary acidic protein ( GFAP), brainderived neurotrophic factor (BDNF), nestin, and factor ∀ antigen. Terminaldeoxynu cleotidyl transferase biotindUTP nick end labeling ( TUNEL) was used to detect apoptosis of cells. R esults In the treated group, the NSS scores and error reaction tmi es in Yelectric maze tests were significantly lower and fewer, the edema and necrosis area and neural scar formationwere less, the num ber ofBrdU/GFAP positive cells, expression ofBDNF, and nestin and ang