siRNAs结合生物芯片的实验设计.pdfVIP

中国试剂网 siRNAs 结合生物芯片的实验设计 Ambion and Applied Biosystems have joined forces to provide a complete convenient, solution for performing gene silencing experiments and validating the results by real-time RT-PCR. Ambions Silencer™ Validated and Silencer Pre-designed siRNAs eliminate the guesswork--and the tedious labwork--associated with siRNA design and testing. Applied Biosystems gene-specific, ready-to-run TaqMan® Gene Expression Assays provide you with a fast, simple way to meas re the efficacy of mRNA knockdown. The ultimate goal of siRNA experiments is to assign gene function, analyze biological pathways, or validate potential drug targets. But before that goal can be realized, the siRNA has to be designed, synthesized, delivered to cells using an optimized protocol, and proven effective. Finally, it is necessary to correlate any induced phenotypic change with the extent of knockdown induced by the siRNA. Ambions Silencer™ Pre-designed siRNAs for 34,000 human, mouse, and rat genes in the NCBI RefSeq database eliminate costly and time-consuming siRNA design, synthesis, and testing steps. Similarly, Applied Biosystems offers TaqMan® Gene Expression Assays for quantitative gene expression analysis of 41,000 human, mouse, and rat genes by real-time PCR. These assays can be used to measure mRNA knockdown for siRNA validation, to optimize transfection, and to correlate phenotype with the extent of knockdown induced by a particular siRNA. Assays to Monitor Gene-specific siRNA Effects siRNAs exert their effects at the mRNA level. Therefore, the preferred assay for siRNA validation and transfection optimization p