SSR分子标记的开发技术研究进展_唐荣华.pdfVIP

西 南 农 业 学 报 2002 15 4 106 Southw est Chin Journ l of Agricultur l Sciences Vol1 15 No14 : 1001- 4829 ( 2002) 04- 0106- 04 SSR 1 2 2 唐荣华 , 张君诚 , 吴为人 ( 11 , 530007; 21 , 350002) : 综述简单重复序列标记( SSR) 的特点及在作物遗传图谱构建品种鉴定及分子标记辅助 种等方面的应用价值重点描 述开发SSR 标记的两种方法的基本原理和主要步骤, 一种是通过克隆酶切片段和大量测序寻找SSR 标记的传统方法, 一种是应用 SAGE 原理不需要克隆的ST MP 技术介绍这两种方法在创建小麦或大豆SSR 标记中的应用它们都能有效地开发可扩增出特 定位点SS R 的PCR 引物 : SS R; 分子标记; 引物开发 : S188 : A Progress in the way to develop SSR molecular marker 1 2 2 TANG Rong-hu , ZHANG Jun-ch eng , WU Wei-ren ( 1. Rese rch Institute of C sh Crops, Gu ngxi Ac demy of Agricultur l Sciences, N nning Gu ngxi 530007, Chin ; 2. College of Crop Science, Fuji n Agriculture Forestry Un iversity, Fuzhou Fuji n 350102,Ch in ) Abstract:T he ch r cteristics of simple sequence repe ts( SSR) nd its import nce in genetic m pping, v riety identifying nd molecul r m rker- ssist nt breeding, etc, re discussed in this rticle. And tw o w ys wh ich c n develop SSR molecul r m rk ers w ith their b sic princ-i ples nd m in steps re lso shown in this rticle. One is the tr dition l w y wh ich needs gre t number of clon ing of enzyme-cut DNA fr gments nd gre t de l of sequencing, the noth er is new w y w hich c n elimin te the cloning step nd c n reduce much more w ork of sequencing by the b sic principle of seri l n lysis of gene ex pression( SAGE) . The utility of these tw o w ys in the development of bre d w he t or soybe n SSR is show n. T he results show ed th t they w ere effective in the designing of PCR primers to