嘉南药理科技大学专题研究计画成果报告.docVIP

嘉南藥理科技大學專題研究計畫成果報告 餵食蛋白酵素對石斑幼苗之生長影響 計畫類別：■個別型計畫 □整合型計畫 計畫編號：CNHR92-01 執行期間：92年1月1日至92年12月31日 計畫主持人：王瑞顯 共同主持人： 計畫參與人員： 執行單位：餐旅管理系 中華民國93年2月12日 嘉南藥理科技大學專題研究計畫成果報告 餵食蛋白酵素對石斑幼苗之生長影響 Effects of survival on Epinephelus sp. larvae fed on protease supplemented diet 計畫編號：CNHR92-01 執行期間：92年1月1日至92年12月31日 主持人: 王瑞顯 嘉南藥理科技大學 餐旅管理系 中文摘要 此實驗在於以一種蛋白酵素添加至基本餌料，然後餵食幼石斑（14天大）以評估其生長與存活。在28天之餵食後，基本餌料組與蛋白酵素添加餌料組之生長與存活率並無顯著之差異，而活豐年蝦餌料餵食幼石斑在存活率（66.6％）或體重（60.2 mg）與皆有最佳之表現。餌料中之蛋白酵素失去活性可能導致幼石斑在28天基本餌料與蛋白酵素添加餌料之餵食試驗間無顯著生長與存活差異。 關鍵詞: 石斑、蛋白酵素、存活率、幼魚苗。 ABSTRACT A bacterial protease was supplemented to a basal diet and fed to the 14 day-old grouper (Epinephelus sp.) larvae for the evaluation of growth and survival. After a 28-day feeding, survivals or body weight of the grouper larvae were no significant difference between the basal diet and protease supplemented diet. Live Artemia nauplii diet fed to the larvae gave the best performance either on final percentage survival (66.6%) or body weight (60.2 mg). The inactivity of protease enzyme in the diet may cause the no significant difference for the growth and survival between protease supplemented diet and basal diet in the 28day feeding trial. Keywords: Grouper larvae, Epinephelus sp., protease, survival. INTRODUCTION The grouper (Epinephelus sp.) is an important recreational and commercial fish species in Taiwan. Due to its high economic value, aquatic farmers have been made to culture this valuable fish species through private and governmental hatcheries in order to enhance populations. Live zooplankton, especially live brine shrimp (Artemia) nauplii, usually provide the best performing diet for rearing the larval stages of fish and crustaceans, since it is easily obtained or cultured, has an appropriate size and is nutritionally adequate (Simpson et al. 1983; Leger et al. 1986). However, various reports have stated that using Artemia nauplii resulted in poor larval rearing, e.g., chlorinated hydrocarbon contamination (O