FMOC柱前衍生高效液相色谱法测定饮料中的牛磺酸.pdfVIP

现代预防医学 2006 年第 33 卷第 3 期　Modern Preventive Medicine , 2006 , Vol 33 , No 3 371　　 【检验方法研究】 FMOC 柱前衍生 - 高效液相色谱法测定 饮料中的牛磺酸 1 1 2 1 王晓莺 , 孙成均 , 杨元 , 张立实 [摘 　要] 　目的 : 建立饮料中牛磺酸的高效液相色谱测定方法。方法 : 以 9 - 氯甲酸芴甲酯 ( FMOC) 为柱前衍生 剂 , 与饮料中的牛磺酸发生衍生化反应后 , 反相高效液相色谱法测定。以甲醇 - 0025 mol/ L 磷酸盐缓冲溶液为流劝相 ( ) ( ) p H = 8 0 , C18柱为分离柱 , 二极管阵列检测器 265 nm 检测。结果 : 本法相对标准偏差为 338 % , 加标回收率范围为 9564 %～1083 % , 检出限为 46 ng 。结论 : 本方法快速、简单、准确度高 , 适合于保健食品类饮料中牛磺酸的测定。 [ 关键词] 　牛磺酸 ; 高效液相色谱法 ; 柱前衍生 [ 中图分类号] R155 5 　　　　 [文献标识码] A 　　　　 [文章编号] 1003 - 8507 (2006) 03 - 0371 - 03 DETERMINATION OF TAURINE IN BEVERAGES BY HIGH PERFORMANCE L IQUID CHROM ATOGRAPHY AFTER DERIVATIZATION WITH 9 - FL UOLENYLMEGHYL CHLOROFORMATE. W A N G Xiaoyin g , S UN Chengjun , YA N G Y uan , et al . West China School of Public Health , Sichuan U niversity , chengdu 610041 Abstract : Objective To establish a high performance liquid chromatographic ( HPLC) method for the determination of taurine in beverages. Methods : After derivatization with FMOC , tarine in beverages was separated and quantitied by HPLC. A mobile phase of methano1 - 0025 mol/ L phosphate buffer (p H = 8 0) , an analytical column of C18 and UV detection wavelength of 265 nm were used in the study. Results : The recoveries ranged from 9564 %～1083 % , RSD was 338 % and detection limit was 46 ng for this method. Conclusion : The method is reliable , accurate and suitable to the determination of taurine in beverages