救必应提取物制备工艺的研究.pdfVIP

广东药学院学报 Journal Pharmaceutical ofGuangdong UniversityApr．2014，30(2) 救必应提取物制备工艺的研究 陈启隆，林伟鑫，林玉婷，王岩 (广东药学院中药学院，广东广州510006) 摘要：目的优选救必应提取物的制备T艺。方法采用单因素法选择提取溶剂：采用币交试验法，以长 梗冬青苷质量分数、浸膏得率为指标，考察提取次数、提取时间、提取溶剂用量3个因素对提取T艺的影 响；采用反相高效液相色谱法测定长梗冬青苷质量分数，烘干法测定浸膏得率。以长梗冬青苷质量分数 为指标优选减压浓缩和干燥T艺。结果救必应最佳提取T艺为：药材加8倍量70％(体积分数)乙醇． 加热回流提取2次，每次1h；在60℃下减压浓缩和干燥。结论该丁艺设计简便、稳定可行，适用于T 业化生产。 关键词：救必应；提取物：制备T艺 中图分类号：R284．2文献标志码：A doi：10．3969／j．issn．1006．8783．2014．02．008 文章编号：1006—8783(2014)02—0156—04 on oftheextractfrom／／exrotunda Studypreparationprocess Thunb． CHEN Weixin，LIN Yan Qilong，LIN Yuting，WANG TraditionalChinese (School Pharmaceutical 5 of Medicine，GuangdongUniversity，Guangzhou10006，China) To the oftheextractfromllexrotunda Abstract：Objectiveoptimize Thunb．．Methods preparationprocess wasusedtoselectthe was to One。wayanalysis solvent．Orthogonaldesigndesigned theamountof investigate timesandtimewiththecontentof and ofextractasindexes．Thecontent solvent，extracting pedunculosideyield of was the ofextractwasdeterminedoven pedunculosideassayedRP-HPLC，and method．The by yield by drying contentof wasusedto the of and underreduced pedunculoside investigateproeessdrying concentrating Thebestextractionconditionswere pressure．Results aS twicewith 70％ethan01． follows：refluxingeight—fold forone houreachtime．The of and underreduced 60