蔷薇科植物果核油性提取物的体内外抗幽门螺杆菌作用.pdfVIP

1 2 3* 蒋锦琴 , 罗冬娇 , 严杰 ( 1. , 310053; 2. , 310018; 3. , 310031) : 观察蔷薇科植物果核油性提取物初提液和提纯液体内外抗幽门 杆菌(H elicobacter pylor,i Hp) 的作用 从胃炎和消化性溃疡患者胃黏膜活检标本中分离H p 临床菌株分别采用二倍平皿稀释法和试管法测定油性提取物初提液 和提纯液对 9 株H p的M IC 和MBC建立H p小鼠感染模型, 检测油性提取物初提液和提纯液体内抗 H p的效果 从 患者胃黏膜活检标本中分离获得 9 株 H p油性提取物初提液和提纯液对上述 Hp 菌株的 M IC 分别为体积分数 0. 1 % 和 90 0. 32% 体积分数为 0. 1 % 油性提取物初提液作用 5 m in 及体积分数为 0. 1 % 的提纯液作用 15 m in 即可杀灭 H p NCTC11 37株临床菌株 75b和 81a株所有感染小鼠胃窦部组织H p SS1株分离培养结果均阳性, 体积分数为 0. 5% 的油性 提取物初提液和提纯液灌小鼠H p分离培养结果均阴性 较低浓度的蔷薇科植物果核油性提取物初提液和提纯液即 有体内抗 H p的作用, 具有进一步开发抗 H p口服液的价值 : 蔷薇科植物果核; 油性提取物; 幽门 杆菌; 抑菌作用; 杀菌作用 : R282. 710. 5 : A : 1007-7 93( 200 ) 05-0355-04 Effects of oiliness extracts from rose family plant fruit core againstH elicoba cter p y lor i in vitro and in vivo 1 2 3* JIANG Jin-qin , LOU Dong-jiao, YAN Jie ( 1. Zhej iang M ed icine College, H angzhou 310053, China; 2. D ep artm ent of Basic M ed icine, H angzhou eachers Colleg e, H angzhou 310018, China; 3. Department of M edicalM icrobiology and Parasitology, College of M ed icine, Zhej iang Un iversity, H angzhou 310031, China) ABSTRACT: OBJECTIVE To determ ine the effects of original and purified oiliness extracts from rose fam ily plant fru it core against H elicobacterpylori in vitro and in vivo. MET ODS C lin icalH. pylori strains were isolated from gastric b iopsy specmi ens of patients w ith gastritis and/ or peptic u lcer. Doub ling agar dilution m ethod and test tube m ethodw ere app lied to detect theM IC and MBC of the orig inal and purified oiliness extracts against the isolates ofH. pylori. H. p ylori infection m ouse model was established to detect the ac- tivities of the original a