玉米ZmPR4启动子的克隆及其在小麦幼胚愈伤组织中的活性分析.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2010, 36(9): 1605−1609 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2010.01605 玉米ZmPR4 启动子的克隆及其在小麦幼胚愈伤组织中的活性分析 王爱云1,2 庄洪涛2,3,** 张增艳2,* 张学文3 杜丽璞2 叶兴国2 1 , 410004; 2 / / , 100081; 3 , 410128 : B73 DNA , PCR , ZmPR4 , AJ969166 100%ZmPR4 ( Ubiquitin) GUS , , ZmPR4 Ubi , , ZmPR4 GUS PCR ZmPR4 , GUS , ZmPR4 : ; ; ; Cloning and Activity Analysis of Zea mays ZmPR4 Promoter in Wheat Imma- ture Embryonic Calli 1,2 2,3,** 2,* 3 2 WANG Ai-Yun , ZHUANG Hong-Tao , ZHANG Zeng-Yan , ZHANG Xue-Wen , DU Li-Pu , and YE 2 Xing-Guo 1 College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China; 2 National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 College of Life Science and Technology, Hunan Agricultural University, Changsha 410128, China Abstract: The ZmPR4 promoter was cloned from genomic DNA of maize inbred line B37 through specific PCR amplification. The promoter cloned had the same sequence as the fragment registered in GenBank under the accession number of AJ969166. Expression vectors driven by ZmPR4 promoter or Ubiquitin promoter were constructed, which harbor GUS gene. The vectors were bombarded into wheat immature embryogenic calli. Histochemical assays of