细胞外信号调节激酶12信号通路在慢性哮喘模型大鼠支气管平滑肌细胞迁移功能变化中地调控作用.pdfVIP

细胞外信号调节激酶12信号通路在慢性哮喘模型大鼠支气管平滑肌细胞迁移功能变化中地调控作用.pdf

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94 Acta Physiologica Sinica, February 25, 2007, 59 (1): 94-102 1/2 * 430030 1/2 (extracellular signal-regulated kinase, ERK1/2) (bronchial smooth muscle cells, BSMCs) BSMCs Western blot RT-PCR ERK1/2 BSMCs ERK1/2 Western blot BSMCs ERK1/2 (9.13±0.87)(4.68±0.59) ERK1/2 (p-ERK1/2)ERK1/2 (0.55±0.05)(0.48±0.04) (n=10, P0.01) ERK1 ERK2 mRNA (1.83±0.24 1.07±0.11)(0.58±0.14 0.51±0.12)(n=10, P0.01) BSMCs (2.9±0.1)ERK1/2 (epidermal growth factor EGF)(5.0±0.2)30 mol/L PD98059 (1.7±0.2)BSMCs PD98059 100 mol/L PD98059 (0.8±0.1) BSMCs (1.9±0.1)EGF (3.1±0.2)30 mol/L PD98059 (1.45±0.2)BSMCs ERK1/2 R 3 2 9 Role of extracellular signal-regulated kinase 1/2 signaling pathway in migration of bronchial smooth muscle cells of chronic asthmatic rats * XIE Min, LIU Xian-Sheng , XU Yong-Jian, ZHANG Zhen-Xiang, BAI Jing, NI Wang, CHEN Shi-Xin Department of Respiratory Medicine, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China Abstract: This work was designed to explore the role of extracellular signal-regulated kinase 1/2 (ERK1/2) signaling pathway in migration of bronchial smooth muscle cells (BSMCs) of chronic asthmatic rats. To make chronic asthma model, Wistar rats underwent ovabumin (OVA) injection and eight-week inhalation. BSMCs were cultured in vitro. The expression of ERK1/2 in BSMCs was analyzed by immunocytochemistry, Western blot and RT-PCR. Migration of BSMCs was detected by both plate test and Boyden cell test. Results showed: (1) With Western blot technique, the ratio of p-ERK1/2 to total ERK1/2 in chronic asthmatic group was obviously higher than that in the control group (0.55±0.05 vs 0.48±0.04, n=10, P0.01). (2) With RT-PCR, the relative A values of ERK1 and ERK2 mRNA in airways of chron

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