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94 Acta Physiologica Sinica, February 25, 2007, 59 (1): 94-102
1/2
*
430030
1/2 (extracellular signal-regulated kinase, ERK1/2)
(bronchial smooth muscle cells, BSMCs)
BSMCs Western blot RT-PCR ERK1/2
BSMCs ERK1/2
Western blot BSMCs ERK1/2 (9.13±0.87)(4.68±0.59)
ERK1/2 (p-ERK1/2)ERK1/2 (0.55±0.05)(0.48±0.04) (n=10, P0.01) ERK1
ERK2 mRNA (1.83±0.24 1.07±0.11)(0.58±0.14 0.51±0.12)(n=10, P0.01)
BSMCs (2.9±0.1)ERK1/2 (epidermal growth factor
EGF)(5.0±0.2)30 mol/L PD98059 (1.7±0.2)BSMCs
PD98059 100 mol/L PD98059 (0.8±0.1)
BSMCs (1.9±0.1)EGF (3.1±0.2)30 mol/L PD98059
(1.45±0.2)BSMCs ERK1/2
R 3 2 9
Role of extracellular signal-regulated kinase 1/2 signaling pathway in migration
of bronchial smooth muscle cells of chronic asthmatic rats
*
XIE Min, LIU Xian-Sheng , XU Yong-Jian, ZHANG Zhen-Xiang, BAI Jing, NI Wang, CHEN Shi-Xin
Department of Respiratory Medicine, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and
Technology, Wuhan 430030, China
Abstract: This work was designed to explore the role of extracellular signal-regulated kinase 1/2 (ERK1/2) signaling pathway in migration
of bronchial smooth muscle cells (BSMCs) of chronic asthmatic rats. To make chronic asthma model, Wistar rats underwent ovabumin
(OVA) injection and eight-week inhalation. BSMCs were cultured in vitro. The expression of ERK1/2 in BSMCs was analyzed by
immunocytochemistry, Western blot and RT-PCR. Migration of BSMCs was detected by both plate test and Boyden cell test. Results
showed: (1) With Western blot technique, the ratio of p-ERK1/2 to total ERK1/2 in chronic asthmatic group was obviously higher than
that in the control group (0.55±0.05 vs 0.48±0.04, n=10, P0.01). (2) With RT-PCR, the relative A values of ERK1 and ERK2 mRNA
in airways of chron
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