超声波提取-液相荧光法测定水果中壬基酚聚氧乙烯醚及其降解产物.pdfVIP

174 2010,VoL31，No．12 食品科学 ※分析检测 超声波提取一液相荧光法测定水果中壬基酚聚 氧乙烯醚及其降解产物 吕岱竹 (中国热带农业科学院分析测试中心，海南海口571101) 摘 要：为建立超声波提取热带水果中环境激素壬基酚聚氧乙烯醚及降解产物壬基酚、液相荧光法测定残留量的 方法，对超声提取的温度、时间、功率进行条件优化，提取液经净化浓缩后，用C18色谱柱进行分离，液相色 谱荧光法进行测定。结果表明：样品中壬基酚聚氧乙烯醚及壬基酚在O．5～50“g／mL范围内线性良好，检出限分 102％和75％～92％。该方法快速灵敏、准确度高，符合 别为O．04mg／kg和0．008mg／kg，方法回收率分别为92％ 残留检测的要求。 关键词：壬基酚聚氧乙烯醚；壬基酚；超声波提取；液相荧光法 Determination and in FruitsLCwith ofAlkylphcnolsAlkylphenolEthoxylatesTropicalby Ultrasonic-assistedExtraction LUDai．zhu and 571 Center,ChineseAcademyofTropicalAgriculturalSciences，Haikou (AnalysisTesting 101，China) Abstract：A basedonultrasonic—assistedextractionWas forthedetermination liquidchromatographic(LC)method presented in and residues fruits．Thedeterminationof themethod ofalkylphenolsalkylphenolethoxylatestropical samplesby developed detection． wasachievedthe onaC,scolumnandfluorescence throughstepsofextmction,condensation,chromatographicseparation conditionsfortheultrasonic—assistedextractionof were curvesfor Optimum samplesinvestigated．Calibrationalkylphenol and bothexhibited overtheconcentrationfromO．5to50u limits linearity range g／mE．Dt优tion ethoxylatesnonylphenol good for 0．008 for