Sequence Analysis of mtDNA COI Gene and.pdf

Agricultural Sciences in China 2005, 4(7) : 533-54 1 http ://www.ChinaAgriS Sequence Analysis of mtDNA COI Gene and Molecular Phylogeny of Different Geographical Populations of Bemisia tabaci (Gennadius) 1,2 1 2 1 1 1 CHU Dong , ZHANG You-jun , CONG Bin , XU Bao-yun , WU Qing-jun and ZHU Guo-ren 1 Ins titu te of Veg etab les and F lowers, Chin ese A cademy of Ag ricultura l Sc iences, B eij ing 100081, P.R.Ch ina 2 Co lleg e of P lant Protection, S heny ang Ag ricultural Un ivers ity, S heny ang 110161, P.R. China Abstract Bemisia tabaci(Gennadius) is a serious pest in many cropping systems worldwide and occurs in different biotypes. The mtDNA COI gene of the 12 Bemisia tabaci (Gennadius) populations from different regions and countries were analyzed. Based on mtDNA COI sequ ences, their biotyp es were characterized and phylogenetic relation ships among these populations were established with the method of UPGMA . The results indicated the genetic similarity between those populations from Beij ing, Zhengzhou, Zaozhuang, Nanj ing, Shanghai, Haikou, and the B-biotype population s from California, Texas, Arizona reached 99.8-100%, which meant the nation-wide infested populations of B.tabaci in China in recent years were B-biotypes. Another population collected from Kunming of Yunnan Province showed very high similarity with Q-biotype B.tabaci from Spain and Morocco, which meant the Kunming population was Q-biotype. This is the first report on the invasion of Q-biotype into China. Key words: Bemisia tabaci (Gennadius), mtDNA COI, Biotype, Phylogenetic tree others have not. B.tabaci biotype B is globally distri- INTRODUCTION buted, while the others are only locally . The nuclear