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Development of a loopmediated isothermal amplification assay for rapid detection of BK virus..pdf

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Development of a loopmediated isothermal amplification assay for rapid detection of BK virus.

JOURNAL OF CLINICAL MICROBIOLOGY, May 2007, p. 1581–1587 Vol. 45, No. 5 0095-1137/07/$08.00 0 doi:10.1128/JCM.01024-06 Copyright © 2007, American Society for Microbiology. All Rights Reserved. Development of a Loop-Mediated Isothermal Ampliﬁcation Assay for Rapid Detection of BK Virus Bipin Raj Bista,1 Chandra Ishwad,1 Robert M. Wadowsky,2 Pradip Manna,3 Parmjeet Singh Randhawa,4 1 5 5 1 1 Gaurav Gupta, Meena Adhikari, Rakhi Tyagi, Gina Gasper, and Abhay Vats * 1 Department of Pediatrics, Children’s Hospital of Pittsburgh, 3705 Fifth Avenue, Pittsburgh, Pennsylvania 15213 ; Department of Pathology, Children’s Hospital of Pittsburgh, 3705 Fifth Avenue, Pittsburgh, Pennsylvania 152132; Department of Molecular Diagnostics, ViraCor Laboratories, 1210 NE Windsor Drive, Lee’s Summit, Missouri 640863; Department of Pathology, University of Pittsburgh Medical Center, 200 Lothrop Street, Pittsburgh, Pennsylvania 152134; and Department of Molecular Virology, Atharva BioSciences, New Delhi 110029, India5 Received 16 May 2006/Returned for modiﬁcation 6 July 2006/Accepted 14 December 2006 Loop-mediated isothermal ampliﬁcation (LAMP) is a novel method for rapid ampliﬁcation of DNA. Its advan- tages include rapidity and minimal equipment requirement. The LAMP assay was developed for BK virus (BKV), which is a leading cause of morbidity in renal transplant