Influence of Daphnia infochemicals on functional traits of Microcystis strains (Cyanobacteria).pdfVIP

Hydrobiologia (2009) 635:147–155 DOI 10.1007/s10750-009-9907-5 PRIMARY RESEARCH PAPER Influence of Daphnia infochemicals on functional traits of Microcystis strains (Cyanobacteria) Ineke van Gremberghe Æ Pieter Vanormelingen Æ Katleen Van der Gucht Æ Antoniya Mancheva Æ Sofie D’hondt Æ Luc De Meester Æ Wim Vyverman Received: 16 January 2009 / Revised: 10 July 2009 / Accepted: 13 July 2009 / Published online: 5 August 2009 Springer Science+Business Media B.V. 2009 Abstract We conducted a laboratory experiment to observed between treatments. Despite this, several investigate the influence of Daphnia infochemicals strains reacted to the infochemicals by changing on growth rate, microcystin production, colony functional trait values. Daphnia culture medium formation and cell size of eight Microcystis strains filtrate and, to a lesser extent, sodium octyl sulphate isolated from two lakes. The strains were character- had a negative influence on the growth rate of half of ized genetically by their 16S-23S rDNA ITS the strains and stimulated microcystin production in sequence. The experiment was composed of four one strain, but the latter effect was not Daphnia- treatments: (1) a control using filtered WC medium, specific as Scenedesmus culture medium filtrate had (2) addition of Scenedesmus obliquus culture med- the same effect. Daphnia culture medium filtrate also ium filtrate, (3) addition of Daphnia magna culture induced colony formation in one strain. Our data medium filtrate and (4) addition of sodium octyl suggest that Daphnia infochemicals generally have a sulphate, a commercially available Daphnia info- weak influence on growth rate, microcystin produc- chemical. Our res