塞莱昔布对呼吸系统炎症的影响.pdfVIP

塞莱昔布对呼吸系统炎症的影响 赵良超 梅佳玮 华玮 上官诚芳 吴安琪 *崔永耀 （上海第二医科大学02 级法七班，*上海第二医科大学生理学教研室 上海 200025 ） [摘要] 目的 观察环氧化酶 2(COX-2)抑制剂对豚鼠呼吸系统炎症的影响。方法 建立哮喘模型，观察整体动物呼吸功能、ELISA法检测血浆中LTs含量、气管及 肺组织HE染色及COX-2免疫组化染色。结果 1）与正常对照组(A)比单纯模型组 (B)气道阻力明显增加，阿司匹林组(C)及塞莱昔布组(D)气道阻力上升幅度较小, HE染色病理变化与其相符。2）与A组比，B、C、D组血浆LTs浓度增加。且C、 D 组无明显差异。3）A 组无 COX-2 表达；B 组 COX-2 大量表达；C 组 COX-2 表达 极少；D 组几乎无 COX-2 表达。结论 特异性 COX-2 抑制剂塞莱昔布不增加体内 白三烯合成，对呼吸系统炎症损伤有保护作用。 [关键词] 塞莱昔布; 环氧化酶2抑制剂; 炎症; 哮喘; 豚鼠 Effects of Inhibitor of Cyclo-oxygenase(COX)-2 on Inflammation of the respiratory System [Abstract] Objective To investigate the effects of Inhibitor of Cyclo-oxygenase(COX)-2 on the inflammation of the spirometry system in guinea-pigs. Methods Replication of the asthmatic model, observe the spirometry fonction in vivo. The level of LTs in serum was measured with ELISA. HE stained was used to observe the pathologic changes of traches and pulmonary tissue, Immunohistochemical analysis was used to detect the expressions of COX-2. Result Compared with the control group(A), we have results as follows: (1) The lung resistance (RL) of the model group(B) were significantly elevated, but the pretreatment with Aspirin (C) and Celebrex (D) were elevated less. (2) The group (C) 、(D) increase in the level of LTs in serum and the rises have no difference 1 obviously.(3)The group (A) doesn’t express COX-2.(B) express a lot, and (C)、(D) express little. Conclution Inhibitor of Cyclo-oxygenase(COX)-2, Celebrex does not increase the level of LTs in vivo, have the effect protective on inflammation of the respiratory system. [key words] Celecoxib ； Inhibitor of Cyclo-oxygenase(COX)-2;