《Effect of IL12 on the proliferation and cytotoxicity of CIK cells to gastric adenocarcinoma cell BGC823》.pdfVIP

Chinese-German J Clin Oncol August 2014, Vol. 13, No. 8, P355–P359 DOI 10.1007/s10330-014-0021-4 Effect of IL-12 on the proliferation and cytotoxicity of CIK cells to gastric adenocarcinoma cell BGC-823* 1, 2 2 2 2 Zhihua Wang , Li Qin , Chunyan Zhang , Yuyan Ma 1 Hefei Fenghuang Tumor Hospital, Hefei 267800, China 2 Cancer Research Institute of Harbin Medical University, Harbin 150081, China Received: 15 June 2014 / Revised: 13 July 2014 / Accepted: 23 July 2014 © Huazhong University of Science and Technology 2014 Abstract Objective: The aim of the study was to evaluate the effect of interleukin-12 (IL-12) on the proliferation and cytotoxicity of cytokine-induced killer (CIK) cells in vitro. Methods: Three different combinations of cytokines, IL-2, IL-12 + IL-2, and IL-12, were used to proliferate CIK cells, adding IFN-γ, IL-1 and CD3 McAb in each one. Phenotype of the cells was analyzed by flow cytometry. The cellular proliferation and cytotoxic activity were determined by cytometry and MTT assay. Results: CIK cells generated by the three methods showed high reproductive activity, and no obviously difference in inducing + + CD3 CD56 cells was found among the three groups. The group of IL-2 + IL-12 evidently enhanced both the proliferation and the cytotoxicity of the CIK cells compared with the other two groups (P 0.05). Conclusion: IL-12 could be used to induce the CIK cells as well as IL-2. CIK cells induced by combining IL-12 with IL-2 had stronger proliferative ability and higher cytotoxicity to tumor cells in vitro, which could be used as a potential anti-tumor adoptive immunotherapy in cl