《Effect of Sodium Chloride and Cadmium on the Growth Oxidative Stress and Antioxidant Enzyme Activities of Zygosaccharomyces rouxii》.pdfVIP

J. Ocean Univ. China (Oceanic and Coastal Sea Research) DOI 10.1007/s11802-014-2123-6 ISSN 1672-5182, 2014 13 (3): 460-466 /xbywb/ E-mail:xbywb@ Effect of Sodium Chloride and Cadmium on the Growth, Oxidative Stress and Antioxidant Enzyme Activities of Zygosaccharomyces rouxii * LI Chunsheng, XU Ying , JIANG Wei, LV Xin, and DONG Xiaoyan College of Food Science and Engineering, Ocean University of China, Qingdao 266003, P. R. China (Received August 2, 2012; revised December 4, 2012; accepted July 16, 2013) © Ocean University of China, Science Press and Spring-Verlag Berlin Heidelberg 2014 Abstract Zygosaccharomyces rouxii is a salt-tolerant yeast species capable of removing cadmium (Cd) pollutant from aqueous solution. Presently, the physiological characteristics of Z. rouxii under the stress of sodium chloride (NaCl) and Cd are poorly under- stood. This study investigated the effects of NaCl and Cd on the growth, oxidative stress and antioxidant enzyme activities of Z. rouxii after stress treatment for 24 h. Results showed that NaCl or Cd alone negatively affected the growth of Z. rouxii, but the growth-inhibiting effect of Cd on Z. rouxii was reduced in the presence of NaCl. Flow cytometry assay showed that under Cd stress, NaCl significantly reduced the production of reactive oxygen species (ROS) and cell death of Z. rouxii compared with those in the absence of NaCl. The activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) of Z. rouxii were significantly −1 enhanced by 2%–6% NaCl, which likely contributed to the high salt tolerance of Z. rouxii. The POD activity was inhibited by 20mg L −1 −1