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Chin Med Sci J Vol. 28, No. 1
March 2013 P. 44-49
CHINESE
MEDICAL SCIENCES
JOURNAL
ORIGINAL ARTICLE
Effect of Nitric Oxide on Esophageal Cancer Cell Line TE-1
1 1 2 2 2
Guo-gui Sun , Wan-ning Hu *, Jun Zhang , Cheng-lin Li , and Cong-rong Yang
1Department of Chemoradiotherapy, Tangshan People’s Hospital Affiliated to
Hebei United University, Tangshan 063001, China
2 Department of Radiology, Fourth Hospital of Hebei Medical University,
Shijiazhuang 050011, China
Key words: nitric oxide; manganese superoxide dismutase; esophagealcancer cells; radio-
sensitization enhancement
Objective To investigate the radiosensitizing effect of nitric oxide (NO) combined with radiation on
esophageal cancer cell line TE-1.
Methods Methyl thiazolyl tetrazolium (MTT) assay was used to assess the effects of NO and radia-
tion on TE-1 cells regarding inhibition of cell proliferation. Flow cytometry was used to examine the effect of
NO and radiation on cell apoptosis and cycle. Reverse transcription polymerase chine reaction and Western
blot were used to evaluete the effect of NO on mRNA and protein expression of manganese superoxide
dismutase (MnSOD).
Results NO inhibited the proliferation of TE-1 cells
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