《Effects of Destruxin A on Hemocytes Morphology of Bombyx mori》.pdfVIP

Journal of Integrative Agriculture 2013, 12(6): 1042-1048 June 2013 RESEARCH ARTICLE Effects of Destruxin A on Hemocytes Morphology of Bombyx mori FAN Ji-qiao, CHEN Xiu-run and HU Qiong-bo College of Resources and Environment, South China Agricultural University, Guangzhou 510642, P.R.China Abstract Destruxin A (DA), a kind of cyclo-hexadepsipeptide isolated from entomopathogenic fungus, Metarhizium anisopliae , is an inhibitor of insect’s immunity. But its mechanism has not been clarified yet. In this study, the effects of DA on morphologic changes of in vivo and in vitro hemocytes of silkworm, Bombyx mori , were investigated by means of inverted phase contrast microscopy (IPCM), fluorescence microscopy (FCM) and environmental scanning electron microscopy (ESEM). The results indicated that DA was cytotoxic to granulohemocytes (GR) and plasmatocytes (PL). The LC50 values of DA against in vitro GR and PL of silkworm were 68.77 and 84.11 μg mL-1, respectively. However, the hemocytes in vivo were more susceptible to DA, although at the extremely low dose of 10 μL of 12.5 μg mL-1 for each insect (i.e., 0.036 μg g-1 body weight, or approximately 0.25 μg mL-1 hemolymph), DA could induce obviously morphologic alterations of hemocytes in vivo. The results imply that there might be some factors in silkworm’s hemolymph, which influence the interaction of DA and hemocytes. Key words: destruxin, toxicity, hemocytes, silkworm Malpiphian tubes (Ruiz-Sanchez et al. 2010a), sali- INTRODUCTION vary glands (Sowjanya and Padmaja 2008) and muscles (Ruiz-Sanchez et al