《Effect of grape proanthocyanidins on tumor growth and angiogenesis in H22 liver cancer xenograft model》.pdfVIP

Chinese-German J Clin Oncol February 2014, Vol. 13, No. 2, P75–P79 DOI 10.1007/s10330-013-1264-1 Effect of grape proanthocyanidins on tumor growth and angiogenesis in H22 liver cancer xenograft model 1 2 3 1 1 1 1 Lili Feng , Jinyi Zhong , Bingxia Liu , Libin Sun , Hongsheng Yu , Yong Qu , Yunyan Luan 1 Department of Oncology, The Affiliated Hospital of Qingdao University, Qingdao 266000, China 2 Institute of Nutrition, Medical College of Qingdao University, Qingdao 266000, China 3 Department of Respiration, Yishui Central Hospital of Linyi City, Linyi 276400, China Received: 3 November 2013 / Revised: 5 December 2013 / Accepted: 5 January 2014 © Huazhong University of Science and Technology 2014 Abstract Objective: The aim of this study was to investigate the effect of grape proanthocyanidins (GPC) on the growth and angiogenesis of hepatocellular carcinoma H22 cells xenograft in mice. Methods: The xenograft model was established using injected subcutaneously H22 cells into the right axilla of the mice. Each group was treated with different doses of GPC and Endostar. All these treatments were maintained for 10 days, and mice were sacrificed. The xenograft tumors in mice were measured. The proliferation activity level of H22 cells was determined by MTT assay, and the levels of vascular endothelial growth factor (VEGF) protein were examined by immunohistochemistry. Results: When treated with 50, 100 and 200 mg/kg of GPC and Endostar, the tumor inhibition rates were 13.17%, 23.37%, 36.15% and 14.71%, respectively. The tumor weight of xenograft was significantly lighter in high GPC group than the control group (P 0.05). The ODs in