《Hepatoprotective and Antioxidant Activity of Rhizome of Podophyllum hexandrum against Carbon Tetra Chloride Induced Hepatotoxicity in Rats》.pdfVIP

Biomed Environ Sci, 2013; 26(3): 209‐221  209 Original Article Hepatoprotective and Antioxidant Activity of Rhizome of Podophyllum * hexandrum against Carbon Tetra Chloride Induced Hepatotoxicity in Rats 1 2 1 1,# Showkat Ahmad GANIE , Bilal Ahmad ZARGAR , Akbar MASOOD , and Mohammad Afzal ZARGAR 1. Department of Biochemistry, University of Kashmir, Srinagar 190006, India; 2. Department of Pharmaceutical Sciences, University of Kashmir, Srinagar JK Abstract  Objective  To test possible antioxidant activity of n‐hexane extract of Podophyllum hexandrum under  in vitro and in vivo conditions.  Methods  The in vitro antioxidant activity was evaluated by the ability of the extract to interact with  the stable free radical DPPH, Superoxide (O ‐), Hydroxyl (OH ), Hydrogen peroxide (H O ) radicals, and ‐ 2 2 2 reducing power ability of the extract was also evaluated. Under in vivo conditions the extract was  evaluated for its hepatoprotective activity by measuring different biochemical parameters, such as  serum  alanine  aminotransaminase,  serum  aspartate  aminotransaminase  and  serum  lactate  dehydrogenase  and  antioxidant  enzymes.  Antioxidant  status  was  estimated  by  determining  the  activities  of  antioxidative  enzymes,  glutathione  reductase  (GR),  glutathione  peroxidase  (GPx),  glutathione‐S‐transferase (GST