人工合成具有白菜或甘蓝细胞质的甘蓝型油菜.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2010, 36(8): 1280−1285 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2010.01280 人工合成具有白菜或甘蓝细胞质的甘蓝型油菜 李 俊 罗莉霞 王 转 李 均 陈坤荣 任 莉 方小平* / , 430062 : , 12 (2n=20)1 (2n=18), , × 2.32%, ×1.16%, , 0.01%MS 10 d , 59.32% , , , 40.53%~88.95% : ; ; ; Resynthesis of Brassica napus with Brassica oleracea or Brassica rapa Cytoplasm ∗ LI Jun, LUO Li-Xia, WANG Zhuan, LI Jun, CHEN Kun-Rong, REN Li, and FANG Xiao-Ping Oil Crops Research Institute of Chinese Academy of Agricultural Sciences / Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Wu- han 430062, China Abstract: Nuclear genome is the primary genetic material, which plays a key role in the individual development and the system evolution of plant. Although relatively independent genetic system in cytoplasm is existed, the characteristics of plant is more often determined by the nuclear genes and sometimes affected by plasmagenes. To compare genetic effect of different plasma- genes of B. napus and study interactions between nuclear gene and cytogene, we artificially synthesized B. napus with B. oleracea or B. rapa cytoplasm by means of hybrid embryo and ovary culture of reciprocal crosses between 12 cultivated varieties of B. rapa and one cultivated variety of B. oleracea var. alboglabra. The average frequencies of hybrid plantlets obtained were 2.32% from ovary culture of B. rapa × B. ol