原创力文档《Identification of Differentially Expressed Genes in the Salivary Gand of Rhipicephalus haemaphysaloides by the Suppression Subtractive Hybridization Approach》.pdfVIP
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Identificationof in
Genesthe Gandof
DifferentiallyExpressed Salivary
the
RhipicephalushaemaphysaloidesSuppression
by
HybridizationApproach
and
XIANG ZHOUJin—lin
Fei—yu，ZHOUYong—zhi
ResearchInstitute，Chinese
KeyLaboratoryofAnimalParasitology,MinistryofAgriculture／ShanghaiVeterinary AcademyofAgricultural
200241，P．R．China
Sciences，Shanghai
Abstract
Forthe of and the fromthe of
salivary
purposescreeninganalyzingdifferentiallyexpressedgenes gland
cDNAlibrariesof fedfemaleticksandfedmaletickswere
haemaphysaloides，twosalivarygland．subtracted partially
female
constructed subtractive totalof247
usingsuppression hybridization(SSH)．Aexpressionsequencetags(ESTS)
and168maleESTswereobtainedfromthetwoSSHcDNAlibraries．Itis that25femaleESTsand44femaleESTs
predicted
7 7 7
containthe5 and3 that53maleESTsand74maleESTscontainthe5 and3
ends，respectively，and
To thesubtractionrateofthetwo cDNA wasusedto 24female
identify SSH libraries．theImPCRmethod test ESTsand
21 male butnot werel
ESTsselected resultsshowedthatthere 3 or
randomlyrepeatedly．The
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