固态发酵高温豆粕制备多肽饲料的最优发酵工艺条件研究.pdfVIP

30 2 Vol. 30 N o. 2 2011 4 SOYBEAN SCIENCE Apr. 2011 1 1 1 2 2 刘晓艳 , 于纯淼, 国立东, 杨国力 , 杨庆余 ( 1. , 150040; 2. , 161006) : , , : 21, 110% , 4% , ( RSA) : 15% , 32 , 73 h, 46. 29% : ; ; ; : TS201 : A : 2011) Optim ization on Solid State Ferm entation Conditions of Producing Polypeptide Feed from H ightemperature SoybeanM eal 1 1 1 2 2 LIU X i oy n , YU Chunm i o , GUO Lidong , YANG Guoli, YANG Q ingyu ( 1. C ollege of Ph rm cy, H eilongji ng Un iversity of Tr dition l Ch inese M edicine, H rb in 150030; 2. Key L bor tory of Processing Agricultur l Produ cts ofH eilongji ng Prov ince, College of Food Scien ce nd E ngineering, Q iqih er Un iversity, Q iqih r 161006, H eilongji ng, Ch in ) A bstract: The optmi l solid st te ferm ent tion cond itions of polypeptide production feed w ith B ac illus subt ilis from hightem per ture soybe n m e lw ere studied in th is p per. The effects ofm edium com position on the y ield of polypeptide were stud ied respectively. The orthogon l expermi entw s designed to ob t in optmi l ferm ent tion m edium, wh ich w ere soybe n m e lwhe t m e l 21, dding 110% w ter nd 4% m ol sses. The effects of ferment tion f ctors on the y ield of polypeptidew ere studied, respectively. The optmi l solid st te ferm ent tion conditions obt ined from response surf ce m ethodology w ere inocu l ted w ith 15% ofB ac illus sub tilis nd ferm en ted t 34 for 72 h, the production r te of soybe n polypeptides cou ld re ch 46. 29% . K ey words: H ightem per ture soybe n m e ;l Polypeptide; B ac illus subt ilis; Solidst te ferm ent tion , 40%~