[PDF] MicroRNA 定量检测方法的研究进展.pdfVIP

HEREDITAS (Beijing) 2010 1 , 32(1): 31―40 ISSN 0253-9772 综述 DOI: 10.3724/SP.J.1005.2010.00031 MicroRNA 定量检测方法的研究进展 1 2 1,3 景花 , 宋沁馨 , 周国华 1. , 210009; 2. , 210009; 3. , 210002 MicroRNA 是一类内源性的非编码小分子RNA, 通过下调蛋白编码基因的表达而对不同的细胞发育过程 起到重要的调控作用。分析组织或细胞样本中microRNA 的表达可为研究这类分子的生物学功能提供重要的信 息。近年来, 研究者发展了许多方法检测不同的生理和病理学过程中microRNA 的表达差异, 并发现microRNA 的异常表达与癌症、神经紊乱和心脏疾病等的发生相关。文章系统地介绍了最新发展的microRNA 定量检测方 法, 详细阐述了基于探针杂交技术的Northern blotting 法、微阵列芯片法、纳米金标记法、桥连同位素标记法, 以 及基于扩增技术的定量PCR 检测法、滚环扩增法、引物入侵法和新一代大规模高通量测序法等, 并对这些方法 的优缺点进行了分析比较。 microRNA; 定量检测; 研究进展 Advances in approaches for the quantitative detection of microRNAs 1 2 1, 3 JING Hua , SONG Qin-Xin , ZHOU Guo-Hua 1. School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China; 2. Department of Pharmaceutical Analysis , China Pharmaceutical University, Nanjing 210009, China; 3. Huadong Research Institute for Medicine and Biotechnics, Nanjing 210002, China Abstract: MicroRNAs (miRNAs) are a class of endogenous non-coding RNAs that play an important role in the control of developmental process of different cells by negative regulation of protein-coding gene expression. Analyzing miRNA expression in tissues or cells can supply valuable information for investigating the biological function of these molecules. Recently, researchers had proposed a number of approaches for analyzing the differences of miRNA expression among dif- ferent physiological or pathological conditions, and found that aberrant expression of miRNA was related to cancers, neu- rological disorders and heart diseases, e