毛霉的产蛋白酶发酵条件优化.pdfVIP

微生物学通报 FEB 20, 2009, 36(2): 193~197 Microbiology © 2009 by Institute of Microbiology, CAS tongbao@ 研究报告 毛霉的产蛋白酶发酵条件优化 郑晓婷 赵新淮* ( 150030) 摘 要: 从发酵豆制品中分离出 1 株产蛋白酶性质优良的毛霉 M2, 并研究 M2 菌株的产蛋白酶条 件。研究优化得出该毛霉菌株的产酶培养基条件为：氮源为大豆分离蛋白, 碳源为葡萄糖, 无机 盐为磷酸二氢钾、氯化钙和氯化镁; 它的适宜的产酶发酵条件为：培养温度为 28°C, 接种量为 2%, 300 mL 三角瓶的装液量为 100 mL, 初始 pH 为 5, 摇床转速为 150 r/min, 培养时间为 48 h; 发酵 液蛋白酶酶活力可达 4.35 U/mL 。凝胶电泳法测定出毛霉所分泌蛋白酶的分子量为 36.4 kD 。 关键词: 毛霉, 蛋白酶, 发酵条件 Optimization of Fermentation Conditions for Proteases Produced by Mucor * ZHENG Xiao-Ting ZHAO Xin-Huai (Key Laboratory of Dairy Science, Ministry of Education , Northeast Agricultural University, Harbin, Heilongjiang 150030, China) Abstract: A strain of Mucor named M2, which could produce protease, was isolated from a traditional fer- mented soybean product. Culture conditions of proteases produced by M2 were studied therefore. The results showed that nitrogen source and carbon source preferred for protease production were soybean protein iso- late and glucose, while inorganic salts preferred were KH PO , CaCl and MgCl . The suitable culture con- 2 4 2 2 ditions for protease production were as follows: culture temperature was 28°C, inoculation volume was 2%, liquid level was 100 mL in 300 mL triangle bottle at pH 5, rotating speed was 150 r/min and culture time was 48 h. The obtained protease activity in culture was about 4.35 U/mL. The protease prod